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作 者:李洁 李丹红 刘丽 孟令锴 吴兆华 LI Jie;LI Danhong;LIU Li;MENG Lingkai;WU Zhaohua(Department of Hematology,Hongqing Hospital Affiliated to Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157011,China;Department of Natural Pharmaceutical Chemistry and Pharmacognosis,School of Pharmacy,Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157011,China)
机构地区:[1]牡丹江医学院附属红旗医院血液科,黑龙江牡丹江157011 [2]牡丹江医学院药学院天然药物化学与生药学教研室,黑龙江牡丹江157011
出 处:《中国中医药现代远程教育》2021年第15期140-141,共2页Chinese Medicine Modern Distance Education of China
基 金:黑龙江省省属高等学校基本科研业务费科研项目【No.2018-KYYWFMY-0069】。
摘 要:目的建立小白花地榆总鞣质的含量测定方法。方法采用比色法,以没食子酸为对照品,测定小白花地榆总鞣质含量。结果以没食子酸计,小白花地榆鞣质浓度在0.986~9.86μg/mL范围内呈良好线性关系(r=0.9996);平均回收率为99.79%,RSD为2.74%(n=8)。结论本实验方法操作简便、准确、稳定性及重复性好,可用于小白花地榆总鞣质的含量测定。Objective To establish a method for the determination of total tannins in Sanguisorba parviflora(Maxim.)Takeda.Methods With Gallic acid as reference substance,the UV spectrophotometer was adopted to determine the content of total tannins.Results With Gallic acid as a standard,Tannins of Sanguisorba parviflora(Maxim.)Takeda showed good linear relationship in the range of 0.986~9.86μg/mL(r=0.9996).The average recovery was 99.79%,with RSD of 2.74%.Conclusion The method is simple,accurate,stable and repeatable and can be used to determine the content of total tannins in Sanguisorba parviflora(Maxim.)Takeda.
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