红光对人毛乳头细胞增殖和分泌细胞因子的影响  

Effect of Red Light on Proliferation of Human Dermal Papilla Cells and Secretion ofCytokines

作　　者：王晓杰 殷文浩 WANG Xiaojie;YIN Wenhao(Department of Dermatology,the First Hospital of Jiaxing,Jiaxing 314000,China)

机构地区：[1]嘉兴市第一医院皮肤科,浙江嘉兴314000

出　　处：《中国皮肤性病学杂志》2021年第9期983-986,共4页The Chinese Journal of Dermatovenereology

基　　金：嘉兴市中医药重点学科-中西医结合皮肤病与性病学(2019XK-C06);嘉兴市第一医院院级课题(2019-YA-53)。

摘　　要：目的探讨红光对毛乳头细胞增殖和分泌血管内皮生长因子(VEGF)及肝细胞生长因子(HGF)的影响。方法运用四甲基偶氮唑蓝(MTT)法检测不同剂量(0.5、1、2、4 J/cm^(2))红光(波长为635 nm)对毛乳头细胞增殖水平的影响。采用蛋白免疫印迹法(Western blot)和实时荧光定量PCR法(RT-PCR)检测不同剂量红光对毛乳头细胞分泌VEGF、HGF的影响。结果红光能显著促进毛乳头细胞增殖,且照光剂量为1 J/cm^(2)为最优剂量;Western blot和RT-PCR结果也显示,红光可上调VEGF、HGF表达水平,且照光剂量为1 J/cm^(2)时,升高水平最为显著。结论红光促进毛乳头细胞增殖的同时,可通过上调VEGF、HGF表达水平,从而促进毛发生长。Objective To investigate the effect of red light on the proliferation of human dermal papilla cells(DPCs)and secretion of vascular endothelial growth factor(VEGF)and hepatocyte growth factor(HGF).Methods The effect of different doses(0.5,1,2,4 J/cm2)of red light(with a wavelength of 635 nm)on the proliferation of DPCs was detected by methyl thiazolyl tetrazolium(MTT).The effect of different doses of red light on the secretion of VEGF and HGF from DPCs was measured by western blot and real-time PCR(RT-PCR).Results The proliferation of DPCs treated with red light was increased,and 1 J/cm2was the optimal dose.The results of Western blot and RTPCR showed that the expression levels of VEGF and HGF were enhanced after treated with red light.The highest level of expression was detected after treatment with red light with 1 J/cm2.Conclusion The red light could promote the proliferation of DPCs,and stimulate the hair growth by up-regulating the VEGF and HGF expression.

关 键 词：红光 毛乳头细胞 血管内皮生长因子 肝细胞生长因子 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]