近红外荧光标记抗体芯片法检测血清中的AFP  

Detection of Alpha-fetoprotein in serum by near-infrared fluorescent labeled antibody chip

作　　者：王志慧 郑刚[1] 许颖原 何月英 WANG Zhihui;ZHENG Gang;XU Yingyuan;HE Yueying(Shanghai Research Engineering Center of Interventional Medical Device,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai Saga Biological Technology Co.,Ltd.,Shanghai 201203,China)

机构地区：[1]上海理工大学上海市介入医疗器械工程研究中心,上海200093 [2]上海萨迦生物科技有限公司,上海201203

出　　处：《光学技术》2021年第4期446-453,共8页Optical Technique

摘　　要：肝癌的发病率和死亡率逐年上升,寻找提高肝癌早期筛查和诊断的有效方法是降低死亡率的重要途径,通过定量检测血清中甲胎蛋白(AFP)是实现肝癌早期筛查和诊断的重要辅助手段。常用的AFP检测方法具有成本高、检测时间长、不适于大规模人群的筛查等缺点,本文采用基于激光共聚焦原理的生物芯片扫描仪和近红外荧光标记抗体芯片相结合的方法,实现AFP的定量检测,并与常用的罗氏电化学发光免疫分析方法检测患者样本中AFP含量进行比较,结果表明,二者之间具有高度相关性。The incidence and mortality of liver cancer are increasing year by year. Finding effective methods to improve early screening and diagnosis of liver cancer is an important way to reduce mortality. Quantitative detection of serum alpha-fetoprotein(AFP) is an important auxiliary method for early screening and diagnosis of liver cancer. The commonly used AFP detection methods have the disadvantages of high cost, long detection time and unsuitable for large-scale population screening. The method of combining a biochip scanner based on the principle of laser confocal and a near-infrared fluorescent-labeled antibody chip is used to achieve the quantitative detection of AFP, and compare it with the commonly used Roche electrochemiluminescence immunoassay method to detect AFP content in patient samples. The results show that there is a high correlation between them.

关 键 词：近红外荧光标记 抗体芯片 AFP 共聚焦 定量检测 

分 类 号：TH773[机械工程—仪器科学与技术] R730.45[机械工程—精密仪器及机械]