Rictor对小鼠基质细胞蜕膜化的影响及其在人自然流产子宫蜕膜组织中的表达  

The Effects of Rictor on Decidualization of Mouse Stromal Cells and Its Expression in Human Spontaneous Abortion Decidual Tissues

作　　者：何立敏 李剑[1] 张悦 刘学庆 HE Limin;LI Jian;ZHANG Yue;LIU Xueqing(Laboratory of the Reproductive Biology,College of Public Health and Management,Chongqing Medical University,Joint International Research Laboratory of Reproduction&Development,Chongqing 400016,China;Department of Cardiology,the First Branch Hospital of the First Affiliated Hospital of Chongqing Medical University,Chongqing 400015,China)

机构地区：[1]重庆医科大学公共卫生与管理学院生殖生物学实验室,教育部生殖与发育国际合作联合实验室,重庆400016 [2]重庆医科大学附属第一医院第一分院心内科,重庆400015

出　　处：《中国细胞生物学学报》2021年第7期1473-1481,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31801247);重庆市自然科学基金重点项目(批准号:csct2019jcyj-msxmX0478)资助的课题。

摘　　要：该研究探讨Rictor及SGK1对小鼠基质细胞蜕膜化的影响及其在人自然流产子宫蜕膜组织中的表达。分离和培养小鼠基质细胞,siRNA沉默小鼠基质细胞Rictor基因并人工诱导蜕膜化,检测Rictor和SGK1的蛋白及mRNA表达情况,并检测蜕膜化标志物Dtprp mRNA的水平;此外,过表达SGK1,检测Dtprp mRNA的水平。选取妊娠8~10周无菌新鲜自然流产及正常人工流产的蜕膜组织标本作为实验研究组(自然流产组,n=17)及正常对照组(正常妊娠组,n=34);分别用Western blot、免疫组织化学、Real-time PCR检测Rictor及SGK1的蛋白和mRNA表达水平。结果表明,沉默Rictor基因后,Rictor及SGK1的蛋白和mRNA表达水平均显著降低,同时蜕膜化指标Dtprp mRNA也显著降低,而过表达SGK1后,蜕膜化指标Dtprp mRNA较前升高且差异有统计学意义。与正常妊娠组相比,Rictor和SGK1的蛋白及mRNA表达水平在自然流产组明显降低。该研究得出,Rictor可通过影响SGK1的表达来抑制基质细胞蜕膜化;Rictor和SGK1在自然流产组的表达均显著低于正常组,从而可能成为流产发生的原因之一。This study was to investigate the effects of Rictor and SGK1 on decidualization of mouse stromal cells and their expression in human spontaneous abortion decidual tissues.Mouse stromal cells were isolated and cultured.siRNA silenced Rictor gene of mouse stromal cells and induced decidualization artificially.The protein and mRNA expressions of Rictor and SGK1 were detected by Western blot and Real-time PCR.The mRNA levels of Dtprp,a marker of decidualization,was detected by Real-time PCR.In addition,SGK1 was overexpressed to detect Dtprp mRNA levels.The decidual tissue samples of sterile fresh spontaneous abortion and normal induced abortion at 8 to 10 weeks of gestation were selected as the experimental study group(spontaneous abortion group,n=17)and normal control group(normal pregnancy group,n=34).The protein and mRNA expression levels of Rictor and SGK1 were detected by Western blot,immunohistochemistry and Real-time PCR.The results showed that the protein and mRNA expression levels of Rictor and SGK1 were significantly decreased after silencing the Rictor gene,and the decidualization index Dtprp mRNA was also significantly decreased.However,after the overexpression of SGK1,the decidualization index Dtprp mRNA was increased compared with before,and the difference was statistically significant.Compared with the normal pregnancy group,the protein and mRNA expression levels of Rictor and SGK1 were significantly decreased in the spontaneous abortion group.In this study,Rictor inhibited stromal cell decidualization by affecting SGK1 expression.The expression of Rictor and SGK1 in the spontaneous abortion group was significantly lower than that in the normal group,which might be one of the causes of abortion.

关 键 词：基质细胞蜕膜化 自然流产 蜕膜组织 Rictor基因 

分 类 号：R714.21[医药卫生—妇产科学]