大肠杆菌O139及其所产志贺样毒素SLT-IIe对猪临床相关指征的影响  

Effect of Escherichia coli O139 and its Shiga-like toxin SLT-IIe on clinical indications in pigs

作　　者：范永超 刘海隆 施晓杰 张倩[1] 冯波[1] 穆祥[1] FAN Yongchao;LIU Hailong;SHI Xiaojie;ZHANG Qian;FENG Bo;MU Xiang(College of Animal Science and Technology,Beijing University of Agricultural/Beijing Key Laboratory of Veterinary Medicine(Traditional Chinese Medicine),Beijing 102206,China;Institute of Animal Husbandry and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China;College of Pharmacy,Minzu University of China,Beijing 100081,China)

机构地区：[1]北京农学院动物科学技术学院/兽医学(中医药)北京市重点试验室,北京102206 [2]海南省农业科学院畜牧兽医研究所,海南海口571100 [3]中央民族大学药学院,北京100081

出　　处：《畜牧与兽医》2021年第8期106-111,共6页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发专项资助项目(2017YFD0501501);北京市生猪产业创新团队项目(BAIC02-2020)。

摘　　要：为了评估大肠杆菌O139及其所产志贺样毒素二型变异体(SLT-IIe)对猪临床相关指征的影响是否一致,试验选取23日龄仔猪15头,随机分为对照组、细菌组、毒素组,每组5只。通过观察和触摸的方法采集各组仔猪的临床症状,通过剖检及HE染色观察各组仔猪脏器的病理变化。结果表明:与对照组相比,毒素组和细菌组呈现面部水肿、共济失调等仔猪水肿病特征性症状,肠腔积液,结肠系膜呈透明胶冻样水肿,胃壁水肿;组织学检查发现模型组小肠绒毛变短,肠黏膜下层及固有层水肿。研究表明,用大肠杆菌O139和SLT-IIe均成功诱导了仔猪水肿病,且用细菌和SLT-IIe诱导的发病猪各项临床指征基本一致。To evaluate the consistency of the effects of Escherichia coli O139 and its Shiga-toxin-type Ⅱ variant(SLT-IIe) on the clinically relevant indications in pigs, 15 piglets aged 23 days were used and randomly divided into the control group, the bacterial group and the toxin group with 5 pigs in each group. The clinical symptoms of the piglets in each group were collected by observation and touch, and the pathological changes in their organs were observed by autopsy and HE staining. The results showed that, compared with the control group, the toxin group and the bacteria group showed facial edema, ataxia and other characteristic symptoms of Piglet Edema Disease, with intestinal cavity effusion, colonic mesentery presenting hyaline gel like edema, and gastric wall edema. The histological examination showed that the small intestinal villi in the model group became shorter, with intestinal mucosa and lamina propria edema. The clinical symptoms and pathological changes in the organs in the two model groups were basically the same. The present results showed that the Escherichia coli O139 and SLT-IIe successfully induced the edema disease in the piglets, and the clinical indications of the animals induced by bacteria and SLT-IIe were generally the same.

关 键 词：大肠杆菌 志贺样毒素 猪水肿病 临床症状 

分 类 号：S853.2[农业科学—临床兽医学]