经皮耳穴迷走神经刺激对功能性消化不良大鼠自主神经功能的影响  

Effects of transcutaneous auricular vagus nerve stimulation on autonomic nervous function in rats with functional dyspepsia

作　　者：侯理伟 荣培晶[1] 李亮[1] 魏玮[2] 方继良[3] 张金铃[1] 王俊英[1] HOU Li-wei;RONG Pei-jing;LI Liang;WEI Wei;FANG Ji-liang;ZHANG Jin-ling;WANG Jun-ying(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences.Beijing 100700,China;Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102;Guangranmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053)

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]中国中医科学院望京医院,北京100102 [3]中国中医科学院广安门医院,北京100053

出　　处：《针刺研究》2021年第8期663-670,共8页Acupuncture Research

基　　金：国家自然科学基金国际合作与交流项目(No.81820108033);国家自然科学基金项目(No.81674072);中国中医科学院2019年中医药“一带一路”国际合作专项(No.GH201912);中国中医科学院针灸研究所基本科研业务费自主选题项目(No.ZZ201915009、ZZ201915012)。

摘　　要：目的:观察经皮耳穴迷走神经刺激(taVNS)对功能性消化不良(FD)大鼠心率变异性(HRV)及自主神经功能的影响,探讨taVNS治疗FD的机制。方法:将SD大鼠随机分为正常组8只,造模组26只。采用碘乙酰胺灌胃法复制FD大鼠模型。将FD大鼠随机分为模型组、taVNS组、taVNS假刺激组、足三里组,每组6只。taVNS组大鼠给予耳穴迷走神经电刺激,taVNS假刺激组仅将电极线固定在耳穴不予电刺激,足三里组予电刺激"足三里",各组每次均干预30 min, 1次/d,连续14 d。采用颈斜方肌肌电(EMG)积分值和腹部撤退反射(AWR)评分评价胃敏感性;HE染色法观察胃窦组织病理形态;记录并分析HRV变化以观察大鼠自主神经功能;ELISA法检测大鼠胃窦组织乙酰胆碱(Ach)的含量;Western blot法检测大鼠胃窦组织Ach受体M3R的表达。结果:与正常组比较,模型组大鼠颈斜方肌EMG积分值和AWR评分升高(P<0.01,P<0.001);胃窦黏膜、肌层均未见糜烂;HRV中高频功率(HF)降低(P<0.05),低频功率(LF)/HF比值增高(P<0.001);胃窦组织中Ach含量及其受体M3R表达降低(P<0.05)。与模型组比较,taVNS组、足三里组大鼠颈斜方肌EMG积分值降低(P<0.01,P<0.001),AWR评分降低(P<0.001,P<0.01,P<0.05);HRV中HF增高(P<0.01,P<0.05),LF/HF降低(P<0.001);胃窦组织中Ach含量及其受体M3R表达增多(P<0.01,P<0.05)。结论:taVNS可提高迷走神经活性,调控自主神经平衡,这可能是taVNS降低FD大鼠的胃敏感性,从而改善胃肠动力的机制之一。在调控迷走神经方面,taVNS和针刺"足三里"具有相似的效应。Objective To observe the effect of transcutaneous auricular vagus nerve stimulation(taVNS) on the autonomic nerve function in a rat model of functional dyspepsia(FD), so as to explore the mechanism of taVNS underlying regulation of FD. Methods SD rats were randomly divided into normal control group(n=8) and FD model group(n=26).The FD model was replicated with iodoacetamide gavage. The FD model rats were randomly divided into model, taVNS, sham-taVNS and Zusanli(ST36) groups, with 6 rats in each group. Rats in the taVNS group received electrical stimulation of auricular concha, while the sham-taVNS group received no electrical stimulation and rats in the ST36 group received stimulation at ST36 for 30 min once daily for 14 consecutive days. Cervical trapezius electromyography score and abdominal withdrawal reflex(AWR) score were used to evaluate gastric sensitivity. Histopathological changes of the gastric antrum tissue were observed under microscope after H.E. staining. Autonomic nerve function in rats was recorded and assessed by heart rate variability(HRV). The content of acetylcholine(Ach) and the expression of Ach receptor M3 R in gastric antrum was detect by ELISA and Western blot, separately. Results Compared with the normal control group, the cervical trapezius electromyography and AWR scores of the model group increased(P<0.01, P<0.001), and there was no erosion in the gastric antral mucosa and muscle layer. The high-frequency power(HF) in HRV decreased(P<0.05), the ratio of low-frequency power/high-frequency power(LF/HF) increased(P<0.001), and the Ach content and its receptor M3 R expression in gastric antrum tissue decreased(P<0.05). Following interventions, the cervical trapezius electromyography and AWR scores decreased(P<0.01,P<0.001, P<0.05), HF in HRV increased and LF/HF decreased(P<0.01,P<0.05,P<0.001), and the content of Ach in gastric antrum tissue and the expression of its receptor M3 R increased(P<0.01, P<0.05) in both taVNS and ST36 groups relevant to the model group. Conclusion taVNS can in

关 键 词：经皮耳穴迷走神经刺激 功能性消化不良 迷走神经 乙酰胆碱 

分 类 号：R245.97[医药卫生—针灸推拿学]