电针预处理对心肌缺血再灌注损伤大鼠下丘脑外侧区神经元电活动的影响  

Effect of electroacupuncture preconditioning on neuronal activities in lateral hypothalamus area of myocardial ischemia reperfusion injury rats

作　　者：张娅婷 蔡荣林[1,2] 崔帅 魏小桐[1] 余情 吴立斌[1] 胡玲 ZHANG Ya-ting;CAI Rong-lin;GUI Shuai;WEI Xiao-tong;YU Qing;WU Li bin;HU Ling(College of Acu-puncture and Moxibustion,Anhui University of Chinese Medicine,Hefei 230012,China;Anhui Key Laboratory of Acupuncture and Moxibustion,Hefei 230038)

机构地区：[1]安徽中医药大学针灸推拿学院,合肥230012 [2]针灸基础与技术安徽省重点实验室,合肥230038

出　　处：《针刺研究》2021年第8期671-678,共8页Acupuncture Research

基　　金：国家自然科学基金项目(No.81774414、82074536、81973757);安徽省高校自然科学研究重点项目(No.KJ2019A0455);安徽省高校优秀拔尖人才培育资助项目(No.gxgwfx2019025)。

摘　　要：目的:观察电针预处理心经经穴对心肌缺血再灌注损伤(MIRI)大鼠下丘脑外侧区(LHA)神经元电活动的影响,探讨电针预处理抗MIRI效应的LHA-小脑顶核(FN)神经环路调控机制。方法:雄性SD大鼠随机分为假手术组、模型组、电针组、损毁+电针组,每组6只。电针组电针"神门""通里",20 min/次,1次/d,共7 d。损毁+电针组在FN注入1 g/L海人酸溶液0.4μL,注射后常规饲养3 d再行针刺预处理干预。采用冠状动脉左前降支结扎法复制MIRI大鼠模型。应用Powerlab多导生理记录仪记录各组大鼠心电图,Plexon多通道采集系统记录各组大鼠LHA神经元放电和场电位(LFP);采用Offline Sorter软件进行聚类分析,以Neuro Explorer软件对神经元放电进行自相关和光栅分析,并分析各组神经元信号的频率和频谱能量。结果:与假手术组比较,模型组大鼠ST段明显抬高(P<0.05),LHA锥体神经元放电次数明显减少(P<0.01),中间神经元放电次数显著增多(P<0.01);与模型组比较,电针组大鼠ST段明显降低(P<0.05), LHA锥体神经元放电次数明显增多(P<0.01);与电针组比较,损毁+电针组大鼠ST段明显抬高(P<0.05),LHA锥体神经元放电次数明显减少(P<0.01)。中间神经元在MIRI模型复制后放电次数均保持较高水平。能量频谱分析显示电针组LFP能量显著低于模型组和损毁+电针组。结论:电针预处理心经经穴可显著提高LHA锥体神经元放电次数并降低局部LFP频谱能量,是其参与抗MIRI效应的机制之一,且这一中枢整合机制可能是通过LHA-FN神经环路实现的。Objective To observe the effect of electroacupuncture(EA) preconditioning of "Shenmen"(HT7)-"Tongli"(HT5) segment of the Heart Meridian on neuronal electrical activities of lateral hypothalamus area(LHA) in rats with myocardial ischemia reperfusion injury(MIRI), so as to investigate its possible mechanism underlying improvement of MIRI. Methods Twenty-four SD rats were randomly divided into sham, model, EA and damage + EA(D+EA) groups, with 6 rats in each group. The MIRI model was established by occlusion of the anterior descending branch(ADB) of the left coronary artery for 30 min, followed by reperfusion for 2 h. In the sham operation group, the ADB was only threaded beneath the artery without ligation. EA(2 Hz, 1 V) was applied to bilateral HT7-HT5 for 20 min, once daily for 7 days. In the D+EA group, FN was injected with 0.4 μL of 1 g/L kainic acid solution. Electrocardiogram(ECG) of the neck-thoracic lead was recorded by using PowerLab. The electrical activities of the LHA region were recorded by the implanted microelectrode array(2×4) and Plexon multi-channel acquisition system. Cluster analysis of neuronal signals was carried out by Offline Sorter software. The discharge waveforms, autocorrelation and cross-correlation of neuronal activities were analyzed by using Neuro Explorer software. Results Compared with the sham group, the ECG-ST height was significantly increased(P<0.05), the discharge frequency of pyramidal neurons was significantly reduced(P<0.01), while the discharge frequency of interneurons was increased(P<0.01) in the model group. After EA treatment, the ECG-ST height was significantly decreased(P<0.05), the discharge frequency of pyramidal neurons was significantly increased(P<0.01) relevant to the model group. In comparison with the EA group, the ECG-ST height in the D+EA group was significantly elevated(P<0.05), whereas the discharge frequency of pyramidal neurons was significantly decreased(P<0.01). The discharge frequency of interneurons was kept increasing after MIRI. Real-time spectr

关 键 词：电针 心经 心肌缺血再灌注 下丘脑外侧区 神经元电活动 

分 类 号：R245.97[医药卫生—针灸推拿学]