温肾散结方对前列腺癌细胞凋亡及迁移侵袭黏附能力的影响及机制  

Effects of Wenshen and Sanjie on apoptosis,migration,adhesion and invasion of prostate cancer cells

作　　者：盛东亚 朱文静[1] 彭煜[1] SHENG Dong-ya;ZHU Wen-jing;PENG Yu(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《时珍国医国药》2021年第5期1028-1031,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81973729;81904070);上海中医药大学附属岳阳中西医结合医院科研项目(2019YYQ34)。

摘　　要：目的研究温肾散结方对细胞凋亡及迁移侵袭黏附能力的影响及机制。方法制备温肾散结方冻干粉后。使用温肾散结方干预PC3细胞,采用CCK8法检测温肾散结对PC3细胞活力的影响,并且计算出50%抑制浓度(IC_(50))。Annexin-V/PI流式细胞术检测细胞凋亡;细胞划痕实验检测细胞的迁移能力;Transwell实验检测细胞的侵袭能力;细胞黏附实验检测细胞的黏附能力;Western blot检测MMP2、MMP9蛋白水平。结果CCK8法检测结果显示温肾散结方显示浓度依赖性抑制PC3细胞的活力,当浓度在64μg/ml时抑制作用达到最大;经过温肾散结方干预后,PC3细胞的凋亡显著增加并且PC3细胞的迁移、侵袭和黏附能力明显下降,MMP2和MMP9蛋白水平显著下降。结论温肾散结方具有抑制PC3细胞的生长,促进其凋亡,抑制其迁移、侵袭和黏附的作用,其作用机制可能与温肾散结方抑制MMP2和MMP9蛋白表达有关。Objective To investigate the effects of Wenshen and Sanjie on the apoptosis,migration,adhesion and invasion of the prostate cancer lines and PC3.Methods PC3 cells were treated with Wenshen and Sanjie at different concentrations,and the cell viability was measured by CCK-8 assay.Apoptosis rate of PC3 cells were determined by flow cytometry.The cell migration,adhesion and invasion abilities were detected by cell scratch test,transwell assay and adhesion assay.The protein expression of matrix metalloproteinase(MMP)-2,MMP-9 was determined by Western blot.Results The results of CCK8 assay revealed wenshen and Sanjie that inhibited the viability of PC3 cells in a dose-dependent manner.The maximal effect was at dose of 64μg/ml.Wenshen and Sanjie promotes the apoptosis of PC3 cells.Wenshen and Sanjie treatment significantly decreased the abilities of migration and invasion of PC3 cells.Moreover,the protein expression of MMP-2 and MMP-9 was dramatically reduced after icariin treatment.Conclusion wenshen and Sanjie inhibits prostate cancer cell viability,promotes the apoptosis of PC3.It inhibits migration,adhesion and invasion by decreasing the protein expression of MMP2 and MMP9.

关 键 词：中药 PC3细胞 前列腺癌 凋亡 转移 侵袭 黏附 

分 类 号：R284.2[医药卫生—中药学]