富含生物活性的可注射硫酸钙骨水泥的研究  

Study on injectable calcium sulfate bone cement rich in bioactivity

作　　者：伦登兴[1] 厉锋[1] 李国雨 吕亚梅 胡永成[2] Lun Dengxing;Li Feng;Li Guoyu;Lyu Yamei;Hu Yongcheng(Department of spinal surgery,Weifang people's Hospital,Weifang 261041,China;Department of bone and soft tissue oncology,Tianjin hospital,Tianjin 300211,China)

机构地区：[1]潍坊市人民医院脊柱外科一区,261041 [2]天津市天津医院骨肿瘤科,300211

出　　处：《中华骨科杂志》2021年第16期1125-1133,共9页Chinese Journal of Orthopaedics

摘　　要：目的探讨富含生物活性的脱钙骨基质(decalcified bone matrix,DBM)对外科级医用硫酸钙的影响,观察不同含量DBM对硫酸钙理化性能影响的变化规律,为制备含有成骨性能的、可注射性的硫酸钙骨水泥提供理论依据。方法按照DBM在硫酸钙复合物的质量比,分为0组、5%组、10%组、20%组、30%组、40%组共6组。充分混合不同含量DBM与外科级硫酸钙制备骨水泥的粉剂。将0.3 g甲基纤维素溶于10 ml的去离子水中,制备浓度为3%的甲基纤维素溶液,作为骨水泥的液剂。按液固比0.4∶1,混合上述骨水泥的粉剂和液剂,搅拌均匀,形成浆体。根据不同的模具,测量并记录DBM/硫酸钙复合物的初凝时间、终凝时间、抗压强度、体外降解率及pH值变化。结果单纯硫酸钙的初凝时间和终凝时间分别为(4.96±0.20)、(5.83±0.12)min。随着DBM的含量的增加,初凝时间和终凝时间增长趋势明显(F=49.275,P<0.05;F=124.859,P<0.05)。单纯硫酸钙(0组)的抗压强度为(23.33±6.35)MPa,40%组抗压强度仅为3.33 MPa。随DBM的含量的增加,抗压强度先增强后降低;5%组、10%组、20%组的DBM对抗压强度影响不大(P>0.05);而30%组、40%组的抗压强度明显降低(t=3.259,P<0.05)。不同含量的DBM能够明显改变硫酸钙复合物的降解速率,30%组和40%组体内完全降解时间仅为10 d;0组30 d的降解速率为63%。在体外降解的任何时间点内,DBM对硫酸钙复合物培养液的pH值均无明显影响,且与单纯硫酸钙的pH变化规律一致。结论随着DBM含量的增加,降解速率逐渐加快,抗压强降低、凝固时间延长,这些不利于可注射型硫酸钙骨水泥的制备。Objective To explore the effect of decalcified bone matrix(DBM)rich in biological activity on surgical-grade medical calcium sulfate,and to observe the change of different content of DBM on the physical and chemical properties of calcium sulfate,which provide theoretical basis for the preparation of calcium sulfate bone cement with osteogenic and injectable properties.Methods DBM with weight content of 0,5%,10%,20%,30%,40% was fully mixed with CSH.Dissolve 0.3 g of methyl cellulose in 10 ml of deionized water to prepare a 3% methyl cellulose solution.Methylcellulose solution was added according to the liquid-solid ratio of 0.4.The mixture was evenly stirred to form slurry,then the degradation rate,compressive strength,setting time and and pH value of calcium sulfate in vitrowas measured.Results The initial setting time and final setting time of calcium sulfate were 4.96±0.20 and 5.83±0.12 min respectively.With the increase of DBM content,the initial setting time and final setting time increased significantly(F=49.275,P<0.05;F=124.859,P<0.05).The compressive strength of pure calcium sulfate is 23.33±6.35 MPa;when the content is 40%,the compressive strength is only 3.33 MPa.With the increase of DBM content,the compressive strength first increased and then decreased;the content of 5%,10%,20%DBM had little effect on the compressive strength(P>0.05),while the compressive strength of 30% and 40% groups decreased significantly(t=3.259,P<0.05).DBM with different contents can significantly change the degradation rate of calcium sulfate complex.When the content of DBM is 30% and 40%,the complete degradation time in vivo is only 10 d,while the degradation rate of calcium sulfate is 63% in 30 d.At any time point in vitro degradation,DBM had no significant effect on the pH value of calcium sulfate complex culture medium,and the change law was consistent with that of pure calcium sulfate.Conclusion With the increase of DBM content,the degradation rate is gradually accelerated,the compressive strength is reduced,and the sett

关 键 词：脱钙技术 硫酸钙 骨水泥成形术 抗压强度 甲基纤维素 

分 类 号：R318.08[医药卫生—生物医学工程]