Compact long-working-distance laser-diode-based photoacoustic microscopy with a reflective objective  

作　　者：Lijun Deng Qi Chen Yang Bai Guodong Liu Lüming Zeng Xuanrong Ji 邓丽军;陈奇;白杨;刘国栋;曾吕明;纪轩荣(State Key Laboratory of Precision Electronics Manufacturing Technology and Equipment,Guangdong University of Technology,Guangzhou 510006,China;Key Laboratory of Optic-Electronics and Communication,Jiangxi Science and Technology Normal University,Nanchang 330038,China)

机构地区：[1]State Key Laboratory of Precision Electronics Manufacturing Technology and Equipment,Guangdong University of Technology,Guangzhou 510006,China [2]Key Laboratory of Optic-Electronics and Communication,Jiangxi Science and Technology Normal University,Nanchang 330038,China

出　　处：《Chinese Optics Letters》2021年第7期56-61,共6页中国光学快报（英文版）

基　　金：supported by the Guangdong Province Introduction of Innovative R&D Team (No.2016ZT06G375);National Natural Science Foundation of China (Nos.11664011,51975131, 11804059, and 51805097);Natural Science Foundation of Jiangxi Province (No.20171ACB20027);2017 Hongcheng Plan of Nanchang Yangshen Electronic Technologies Co.,Ltd。

摘　　要：Photoacoustic microscopy(PAM) has quickly developed into a noninvasive biomedical imaging technique to achieve detection, diagnosis, and monitoring.Compared with Q-switched neodymium-doped yttrium aluminum garnet or optical parametric oscillator lasers, a low-cost and small-size laser diode(LD) used as an alternative light source is conducive to achieving the miniaturization and integration for preclinical transformation.However, the LD’s low peak output power needs the high numerical aperture objective to attain tight focus, which limits the working distance(WD) of the system in only2–3 mm, resulting in not achieving the backward coaxial confocal mode.Here, we present a compact visible LD-based PAM system with a reflective objective to achieve a 22 mm long WD and a 10 μm lateral resolution.Different depth subcutaneous microvascular networks in label-free mouse ears have successfully reappeared in vivo with a signal-to-noise ratio up to14 d B by a confocal alignment.It will be a promising tool to develop into a handy tool for subcutaneous blood vessel imaging.

关 键 词：photoacoustic microscopy long working distance laser diode reflective objective 

分 类 号：TH776[机械工程—仪器科学与技术]