硫化氢对白桦悬浮细胞中IAA·ABA和次生代谢物累积的影响  

The Effect of H_(2)S on the Accumulation of Indoleacetic Acid(IAA),Abscisic Acid(ABA)and Secondary Metabolites in Birch Suspension Cells

作　　者：叶子诺 翟明霞 周文洋[1] 肖婉莹 詹亚光[1] 范桂枝[1] YE Zi-nuo;ZHAI Ming-xia;ZHOU Wen-yang(College of Life Sciences,Northeast Forestry University,Harbin,Heilongjiang 150040)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2021年第17期117-121,127,共6页Journal of Anhui Agricultural Sciences

基　　金：东北林业大学大学生创新创业训练计划项目(2019102-25504);黑龙江省自然科学基金(C2016005)。

摘　　要：[目的]探究硫化氢(H_(2)S)对白桦悬浮细胞中吲哚乙酸(IAA)、脱落酸(ABA)以及多酚和三萜等次生代谢物累积的影响。[方法]采用酶联免疫吸附法、实时荧光定量PCR和比色法等分析IAA和ABA含量及其关键酶基因表达水平、黄酮和三萜等次生代谢物含量。[结果]H_(2)S供体硫氢化钠(NaHS)处理对白桦悬浮细胞中IAA、ABA的累积存在浓度和时间效应,其中1.0 mmol/L H_(2)S供体NaHS处理12 h时ABA含量达最高值,比对照增加了196.46%,而IAA含量在0.1 mmol/L H_(2)S供体NaHS处理3 h时达最高值,比对照增加了5.55%。ABA或H_(2)S处理后白桦悬浮细胞中次生代谢产物积累呈增加趋势,而IAA处理则相反。1.0 mmol/L H_(2)S供体NaHS与不同浓度的IAA和ABA分别处理白桦悬浮细胞24 h后,不同程度地提高了白桦悬浮细胞中多酚、黄酮、三萜含量。其中,1.0 mmol/L ABA与NaHS共同处理后白桦悬浮细胞中多酚、黄酮、三萜含量均达到最大值,分别比NaHS单独处理增加了17.89%、14.74%和8.87%。[结论]H_(2)S处理促进白桦悬浮细胞中IAA、ABA和次生代谢物的累积,H_(2)S与IAA或ABA共同处理可进一步促进次生代谢产物的累积。[Objective]The purpose of this study was to clarify the effect of hydrogen sulfide(H_(2)S)on the accumulation of indoleacetic acid(IAA),abscisic acid(ABA)and secondary metabolites in birch(Betula platyphylla Suk.)suspension cultures.[Method]The colorimetric method,real-time quantitative PCR and enzyme-linked immunosorbent assay were used to analyze the contents of flavones,polyphenols,triterpenes,IAA and ABA,and the gene expression level of IAA and ABA-related key enzyme.[Result]The results showed the concentration and time effects of H_(2)S donor sodium sulfide(NaHS)on the accumulation of IAA and ABA in the birch suspension cells.Among the cells,the highest level of ABA was at 1.0 mmol/L H_(2)S donor NaHS after 12 h treatment,with increasing rates of 196.46%compared to the control.And the highest level of IAA was at 0.1 mmol/L H_(2)S donor NaHS after 3 h treatment,with increasing rates of 5.55%compared to the control.The accumulation of secondary metabolites in the birch suspension cells showed an increasing trend after ABA or H_(2)S treatment,while the opposite was observed after IAA treatment.Treatment on birch suspension cell with 1.0 mmol/L H_(2)S donor NaHS and different concentrations of IAA and ABA for 24 h increased the polyphenols,flavones and triterpenes levels,respectively.Polyphenols,flavonoids and triterpenes reached maximum levels after combined treatment with 1.0 mmol/L ABA and NaHS;the levels increased by 17.89%,14.74%and 8.87%,compared to NaHS treatment,respectively.[Conclusion]H_(2)S treatment induced the synthesis of IAA and ABA and the accumulation of secondary metabolites in the birch suspension cells.Co-treatment with H_(2)S and IAA or ABA respectively,could further promote the accumulation of secondary metabolites.

关 键 词：H 2S 白桦 IAA ABA 次生代谢产物 

分 类 号：S792.153[农业科学—林木遗传育种]