纳帕海高原湿地噬藻体g20基因系统发育多样性分析  

Phylogenetic diversity of cyanophages g20 gene in Napahai plateau wetland in China

作　　者：王粟萍 陈学梅 魏云林[1] 张琦[1] 季秀玲[1] WANG Suping;CHEN Xuemei;WEI Yunlin;ZHANG Qi;JI Xiuling(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《微生物学通报》2021年第8期2574-2582,共9页Microbiology China

基　　金：国家自然科学基金(31860147,31700324)。

摘　　要：【背景】噬藻体是感染蓝藻的病毒,是水生系统的重要组成部分。它们对宿主种群死亡率有重要影响,是控制蓝藻水华生消的潜在因子,对蓝藻群落结构的调控具有重要意义。大量研究揭示了海洋和淡水环境中噬藻体的高度多样性,但目前对高原湿地中噬藻体的多样性知之甚少。【目的】阐明我国纳帕海高原湿地噬藻体g20基因的遗传多样性,为进一步开展高原湿地微生物资源及其生态功能研究提供理论基础。【方法】采集雨季的水体样品,以衣壳蛋白基因g20为标记基因,利用特异性引物Cps1和Cps8对其进行PCR扩增,得到26条不同的g20基因有效序列,并将其与其他生境中g20基因序列进行主坐标分析和系统发育分析。【结果】与其他海洋和淡水的噬藻体序列相比,纳帕海高原湿地中噬藻体的序列与其他稻田序列更为相近;但也存在部分序列单独聚簇,这可能为纳帕海高原湿地中独有的噬藻体类型。【结论】表明该地区的噬藻体较丰富,并具有一定的独特性。[Background]Cyanophages,are viruses infecting cyanobacteria and a potential factor to control the growth and elimination of cyanobacteria blooms,which are of great significance to the regulation of cyanobacteria community structure.A large number of studies previously reported have revealed the high diversity of cyanophages in marine and freshwater environments,however,the knowledge of cyanophages in wetlands is scant.[Objective]It is necessary to clarify the genetic diversity of cyanophages gene g20 in Napahai plateau wetland in China,and provide a theoretical basis for further research on microbial resources and ecological functions of plateau wetlands.[Methods]The water samples were collected during the rainy season.The capsid protein gene g20 as marker gene was amplified by PCR with specific primers Cps1/Cps8 in this study,and 26 different effective sequences of gene g20 were obtained.The principal coordinates analysis and phylogenetic analysis for gene g20 sequences from above and other ecological environment were performed.[Results]The phytophagy sequences in Napahai plateau wetland were more closely related to rice field sequences compared with other marine and freshwater phytophagy sequences.However,some of the sequences were clustered separately,they may be unique type of cyanophages in Napahai plateau wetland.[Conclusion]It shows that cyanophages is abundant and show unique in Napahai plateau wetland.

关 键 词：高原湿地 g20基因 噬藻体 

分 类 号：Q943[生物学—植物学]