枯草芽孢杆菌表达和分泌异源蛋白的研究进展  

Advances in heterologous protein expression and secretion of Bacillus subtilis

作　　者：王杰 王晨 杜燕 徐晶玉 班睿 WANG Jie;WANG Chen;DU Yan;XU Jingyu;BAN Rui(Key Laboratory of Systems Bioengineering of Ministry of Education,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300308,China)

机构地区：[1]天津大学化工学院,系统生物工程教育部重点实验室,天津300308

出　　处：《微生物学通报》2021年第8期2815-2826,共12页Microbiology China

基　　金：国家自然科学基金(31470208)。

摘　　要：枯草芽孢杆菌是一种广泛应用于基础研究和工业生产的重要模式菌株,具有无致病性、蛋白分泌能力强、遗传背景清晰等多种优势,是生产异源蛋白的理想宿主。目前已有诸多异源蛋白在枯草芽孢杆菌中实现表达和分泌,其中包括淀粉酶、β-半乳糖苷酶和蛋白酶等有价值的工业酶。本文从异源蛋白表达和分泌的关键步骤出发,总结了枯草芽孢杆菌生产异源蛋白的传统策略和最新技术。除此之外,分析了当前研究存在的瓶颈并对如何提高异源蛋白产量提出了新的建议和策略。Bacillus subtilis is an important model strain widely used in basic research and industrial production,which has many advantages such as non-pathogenicity,the strong capability of secreting proteins,clear genetic background,so it is an ideal host for expressing and secreting heterologous proteins.At present,there are many heterologous proteins that have been expressed and secreted in Bacillus subtilis,which includes valuable proteins such as amylase,beta-mannanase and protease.Based on the key steps of the expression and secretion of heterologous protein,this paper summarizes the traditional strategies and the latest technologies for heterologous protein production in Bacillus subtilis.In addition,we analyze the difficulties in the present research and propose new suggestions and strategies for the improvement of the production of heterologous protein.

关 键 词：枯草芽孢杆菌 异源蛋白 表达分泌系统 菌株改造策略 限制因素 

分 类 号：Q93[生物学—微生物学] Q78