子宫内膜异位症患者体内氧化应激状态及其与IVF-ET的相关性研究  

Study of oxidative stress status in patients with endometriosis and its correlation with IVF-ET

作　　者：高子轩[1] 罗小婉[1] 栾峰[1] 孙广范[1] 刘佳杰[2] 尚雪[1] GAO Zixuan;LUO Xiaowan;LUAN Feng;SUN Guangfan;LIU Jiajie;SHANG Xue(Department of Gynecology,Zhongshan Boai Hospital,Guangdong Zhongshan 528400,China;Reproductive center,Zhongshan Boai Hospital,Guangdong Zhongshan 528400,China)

机构地区：[1]中山市博爱医院妇科,广东中山528400 [2]中山市博爱医院生殖中心,广东中山528400

出　　处：《中国妇幼健康研究》2021年第8期1219-1223,共5页Chinese Journal of Woman and Child Health Research

基　　金：中山市社会公益科技研究资助项目(2019B1026)。

摘　　要：目的探讨氧化应激标记物在子宫内膜异位症患者中的浓度及其与体外受精-胚胎移植(IVF-ET)的相关性。方法选择2018年7月至2020年6月就诊于中山市博爱医院妇科的子宫内膜异位症术后不孕症患者50例设为观察组,选择同期治疗的输卵管不孕者20例为对照组。两组患者均接受体外受精-胚胎移植(IVF-ET),采用酶联免疫吸附试验测定两组患者血清和卵泡中液活性氧(ROS)、丙二醛(MDA)、总抗氧化能力(TAC)浓度,并分析内异症患者血清氧化应激标记物与IVF-ET指标的相关性。结果观察组血清和卵泡液中ROS、MDA浓度均显著高于对照组(t值分别为5.892、3.323、5.993、3.021,P<0.05),而TAC浓度均显著低于对照组(t值分别为4.063、3.992,P<0.05)。观察组成熟卵率、受精率均显著低于对照组(χ^(2)值分别为22.858、15.338,P<0.05),而两组平均获卵数比较差异无统计学意义(t=0.726,P>0.05),优质胚胎率、种植率、临床妊娠率比较差异均无统计意义(χ^(2)值分别为0.360、0.012、0.092,P>0.05)。Pearson相关性分析结果表明子宫内膜异位症患者血清ROS、MDA均与成熟卵率、受精率呈显著负相关(r值分别为-0.783、-0.813、-0.815、-0.791,P<0.05),而血清TAC均与成熟卵率、受精率呈显著正相关(r值分别为0.768、0.774,P<0.05),血清氧化应激标记物与其他IVF-ET指标均无显著相关性(r值介于0.131~0.493之间,P>0.05)。结论子宫内膜异位症患者体内氧化应激反应增加,而抗氧化应激能力降低,此种变化导致了患者接受IVF-ET时成熟卵率、受精率下降,值得临床关注。Objective To investigate the concentration of oxidative stress markers in patients with endometriosis and their correlation with in vitro fertilization-embryo transfer(IVF-ET). Methods A total of 50 infertile endometriosis patients treated in the department of gynecology of Zhongshan Boai Hospital from July 2018 to June 2020 were selected as the observation group, and 20 infertile tubal patients treated during the same period were selected as the control group. Patients in the two groups received IVF-ET,and the concentrations of reactive oxygen species(ROS),malondialdehyde(MDA) and total antioxidant capacity(TAC) in serum and follicles between the two groups were determined by enzyme linked immunosorbent assay, and the correlation between serum oxidative stress markers and IVF-ET indexes in patients with endometriosis was analyzed. Results The concentrations of ROS and MDA in serum and follicular fluid in observation group were significantly higher than those in control group(t=5.892,3.323,5.993 and 3.021,respectively, P<0.05),while the concentrations of TAC in observation group were significantly lower than those in control group(t=4.063 and 3.992,respectively, P<0.05). The mature egg rate and fertilization rate of the observation group were significantly lower than those of the control group(χ^(2)=22.858 and 15.338,respectively, P<0.05),but there was no statistical significance in the average number of eggs obtained between the two groups(t=0.726,P>0.05),and there was no statistical significance in the rate of high-quality embryo, implantation rate and clinical pregnancy rate between the two groups(χ^(2)=0.360,0.012 and 0.092,respectively, P>0.05). Pearson correlation analysis results showed that serum ROS,MDA of endometriosis patients were significantly negatively correlated with mature egg rate and fertilization rate(r=-0.783,-0.813,-0.815 and-0.791,respectively, P<0.05),but serum TAC was significantly positively correlated with mature egg rate and fertilization rate(r=0.768 and 0.774,respectively, P<0.05).

关 键 词：氧化应激标记物 子宫内膜异位症 体外受精-胚胎移植 相关性 

分 类 号：R711.71[医药卫生—妇产科学]