脂多糖对鲤肠上皮细胞转录组模式的调控分析  

Comprehensive Transcriptome Analysis of Intestinal Epithelial Cells of Cyprinus carpio Exposed to Lipopolysaccharide

作　　者：陈建军[1] 赵怡迪 曹香林 CHEN Jian-jun;ZHAO Yi-di;CAO Xiang-lin(College of Life Science,Henan Normal University,Xinxiang 453007;College of Fisheries,Henan Normal University,Xinxiang 453007)

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]河南师范大学水产学院,新乡453007

出　　处：《生物技术通报》2021年第8期213-220,共8页Biotechnology Bulletin

基　　金：河南省重点科技攻关计划项目(192102110195,152102210081)。

摘　　要：旨为探究脂多糖对鲤肠上皮细胞相关基因及功能的影响。本研究以鲤肠上皮细胞为试验对象,正常处理为对照组,脂多糖处理为实验组,对处理24 h的两组鲤肠上皮细胞进行转录组测序。转录组测序结果共获得44.77G高质量数据,其中,近81.83%的数据能够比对到鲤基因组。利用DESeq软件分析,与对照组相比,脂多糖处理可诱导产生差异表达基因(differentially expressed genes,DEG)590个,其中303个表达上调,287个表达下调。Gene Ontology(GO)功能富集分析发现,DEG在细胞过程、单有机体过程、代谢过程、细胞、细胞组分、细胞器、结合和催化活性功能亚类中所占比例最高。Kyoto Encyclopedia of Genes and Genomes(KEGG)通路途径分析发现,DEG显著富集于细胞周期、自噬、凋亡途径。并利用实时荧光定量PCR对随机挑选的10个差异基因进行验证,结果表明转录组数据可靠。该研究通过转录组测序技术,全面、快速地获取脂多糖暴露于鲤肠上皮细胞过程中的所有转录本信息,系统地证明脂多糖阻滞了鲤肠上皮细胞周期,诱导自噬,引起细胞凋亡,为脂多糖调控鲤肠上皮细胞分子机制提供了重要的理论依据。This work aims to explore the effect of lipopolysaccharide(LPS)on the genes and functions of intestinal epithelial cells in Cyprinus carpio.The intestinal epithelial cells of C.carpio were taken as the research object,normal treatment was as the control group,and lipopolysaccharide treatment was as the experimental group.Transcriptome sequencing was performed on the two groups of the intestinal epithelial cells of C.carpio treated for 24 h.A total 44.77G of high-quality data from transcriptome sequencing results were obtained,of which,nearly 81.83%data could be aligned to the C.carpio genome.Analyzed using the DESeq software and compared with the control group,590 differentially expressed genes(DEG)were induced by LPS treatment.Among them,303 were up-regulated and 287 were down-regulated.Gene Ontology(GO)functional enrichment analysis showed that DEG accounted for the highest proportion in cellular processes,single organism processes,metabolic processes,cells,cell part,organelle,binding and catalytic activity functional subclasses.The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that DEG was significantly enriched in cell cycle,autophagy and apoptosis pathways.The 10 randomly selected differential genes were verified by quantitative real-time PCR,and the results showed that the transcriptome data were reliable.In this study,transcriptome sequencing technology was used to comprehensively and rapidly obtain all transcriptome information during LPS exposure to C.carpio intestinal epithelial cells,and to systematically prove that LPS blocked the cell cycle of C.carpio intestinal epithelial cells,induced autophagy and apoptosis,thus providing an important theoretical basis for the molecular mechanism of LPS regulation in C.carpio intestinal epithelial cells.

关 键 词：脂多糖 鲤 肠上皮细胞 转录组测序 实时荧光定量PCR 

分 类 号：S943[农业科学—水产养殖]