TRIF在血栓闭塞性脉管炎患者血管组织中的表达水平及临床意义  

The expression and clinical significance of TRIF in the vascular tissues of patients with thromboangiitis obliterans

作　　者：尹江燕[1] 郭轶[2] YIN Jiangyan;GUO Yi(Department of Ultrasonic,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Department of General Surgery,Affiliated Central Hospital of Chongqing University/Chongqing Emergency Medical Center,Chongqing 400014,China)

机构地区：[1]重庆医科大学附属第一医院超声科,重庆400016 [2]重庆大学附属中心医院/重庆市急救医疗中心普外科,重庆400014

出　　处：《国际检验医学杂志》2021年第17期2090-2093,共4页International Journal of Laboratory Medicine

基　　金：重庆市科技局自然科学基金面上项目(cstc2020jcyj-msxm3181)。

摘　　要：目的探讨β干扰素Toll样受体结构域衔接蛋白(TRIF)在血栓闭塞性脉管炎(TAO)患者血管组织中的表达水平及临床意义。方法取因TAO行截肢手术的26例患者(TAO组)和因非血管疾病行截肢手术的18例患者(对照组)的血管组织,采用反转录PCR(RT-PCR)及Western blot检测两组患者血管组织中的TRIF表达水平并进行组间比较。进一步采用免疫荧光共聚焦技术观察TRIF在TAO患者血管组织中的表达特点。结果TAO患者血管组织中TRIF的mRNA和蛋白表达水平明显高于对照组(P<0.05)。并且,在TAO患者中,TRIF主要表达于血管平滑肌细胞。结论TRIF的表达与TAO的起病关系密切,TRIF信号通路诱导的炎性反应可能是TAO发病的重要因素之一。Objective To investigate the expression of Toll/IL-1 receptor domain containing adaptor inducing beta-interferon(TRIF)in vascular tissues of patients with thromboangiitis obliterans(TAO)and its clinical significance.Methods The vascular tissues of 26 patients who underwent amputation due to TAO(TAO group)and 18 patients who underwent amputation due to non-vascular disease(control group)were collected,and the two groups were detected by using RT-PCR and Western blot.The expression levels of TRIF in patients′vascular tissues were compared between the two groups.The immunofluorescence confocal technique was further used to observe the expression characteristics of TRIF in the vascular tissues of TAO patients.Results In patients with TAO,the mRNA and protein expressions of TRIF were much higher than that of in patients without vascular diseases(P<0.05),and the expression of TRIF was mainly in smooth muscle cells of blood vessel.Conclusion The expression of TRIF is closely related to the development of TAO,and the inflammatory response induced by the TRIF signal pathway may be one of the important factors in the onset of TAO.

关 键 词：β干扰素Toll样受体结构域衔接蛋白 血栓闭塞性脉管炎 炎性反应 

分 类 号：R446.8[医药卫生—诊断学]