骨髓间充质干细胞对小鼠缺血-再灌注急性肾损伤中IL-10和TNF-α表达的影响  

Effect of bone marrow mesenchymal stem cell on the expression of IL-10 and TNF-αin mice with ischemia-reperfusion acute kidney injury

作　　者：蔺晨雨 陈文 马锡慧 孔祥瑞 樊文梅 韩永 肖漓 石炳毅 Lin Chenyu;Chen Wen;Ma Xihui;Kong Xiangrui;Fan Wenmei;Han Yong;Xiao Li;Shi Bingyi(Hebei North University,Zhangjiakou 075000,China;不详)

机构地区：[1]河北北方学院,河北张家口075000 [2]中国人民解放军总医院第八医学中心呼吸与危重症医学部研究所北京市器官移植与免疫调节重点实验室

出　　处：《器官移植》2021年第5期563-570,共8页Organ Transplantation

基　　金：军队医学科技青年培育计划(18QNP035)。

摘　　要：目的探讨骨髓间充质干细胞(BMSC)对小鼠缺血-再灌注急性肾损伤(IR-AKI)过程中白细胞介素(IL)-10和肿瘤坏死因子(TNF)-α表达的影响。方法将小鼠随机分为假手术组(对照组)、缺血-再灌注损伤组(IRI组)和BMSC治疗组(BMSC组),每组6只。检测各组小鼠肾功能及病理学改变;检测各组小鼠肾组织细胞凋亡情况;检测各组小鼠血清IL-10和TNF-α的表达水平。将小鼠BMSC随机分为对照组和缺氧复氧组(IRI组),检测各组细胞上清IL-10和TNF-α的表达水平。结果对照组小鼠肾组织结构正常,IRI组肾组织结构损伤严重,BMSC组损伤较轻。与对照组比较,IRI组和BMSC组肾组织损伤评分较高;与IRI组比较,BMSC组小鼠肾组织损伤学评分较低(均为P<0.05)。与对照组比较,IRI组小鼠血清肌酐(Scr)和血尿素氮(BUN)水平升高,BMSC 组BUN 水平升高;与IRI 组比较,BMSC 组小鼠Scr 和BUN 水平下降(均为P<0.05)。IRI 组肾组织凋亡细胞数量多于BMSC 组和对照组,BMSC 组凋亡细胞数量多于对照组(均为P<0.05)。与对照组比较,IRI 组小鼠血清IL-10 和TNF-α 水平均升高,BMSC 组小鼠血清TNF-α 水平下降,IL-10 水平升高;与IRI 组比较,BMSC 组小鼠血清IL-10 和TNF-α 水平均下降(均为P<0.05)。IRI 组细胞上清IL-10、TNF-α 水平与对照组比较,差异均无统计学意义(P=0.080、0.627)。结论 BMSC 输注可降低肾IRI 及炎症反应,其机制可能是通过抑制TNF-α 表达,而非促进IL-10 的表达。Objective To evaluate the effect of bone marrow mesenchymal stem cell(BMSC)on the expression of interleukin(IL)-10 and tumor necrosis factor(TNF)-αin mice with ischemia-reperfusion acute kidney injury(IRAKI).Methods All mice were randomly divided into the sham operation group(control group),ischemia-reperfusion injury group(IRI group)and BMSC treatment group(BMSC group),with 6 mice in each group,respectively.The renal function and pathological changes of mice were detected.The cell apoptosis of renal tissues of mice was determined.The expression levels of serum IL-10 and TNF-αof mice were quantitatively measured.The mouse BMSC was randomly divided into the control and hypoxia-reoxygenation groups(IRI group),and the expression levels of IL-10 and TNF-αin cell supernatant were determined.Results The renal structure of mice was normal in the control group,severe damage was observed in the IRI group,and mild damage occurred in the BMSC group.Compared with the control group,the renal tissue injury scores were significantly higher in the IRI and BMSC groups(both P<0.05).Compared with the IRI group,the renal tissue injury score was significantly lower in the BMSC group(P<0.05).Compared with the control group,the levels of serum creatinine(Scr)and blood urea nitrogen(BUN)were remarkably up-regulated in the IRI group,and the level of BUN was significantly up-regulated in the BMSC group(all P<0.05).Compared with the IRI group,the levels of Scr and BUN were significantly down-regulated in the BMSC group(both P<0.05).In the IRI group,the quantity of apoptotic cells in the renal tissues was considerably higher than those in the BMSC and control groups,and the quantity of apoptotic cells in the BMSC group was significantly higher than that in the control group(all P<0.05).Compared with the control group,the levels of serum IL-10 and TNF-αwere significantly up-regulated in the IRI group,whereas the level of serum TNF-αwas significantly down-regulated and the level of serum IL-10 was significantly up-regulated in the BMSC g

关 键 词：肾移植 骨髓间充质干细胞 缺血-再灌注损伤 急性肾损伤 炎症反应 白细胞介素(IL)-10 肿瘤坏死因子(TNF)-α 血清肌酐 血尿素氮 

分 类 号：R617[医药卫生—外科学] R364.5[医药卫生—临床医学]