基于网络药理学探讨淫羊藿补肾作用对多囊卵巢综合征的影响  

Mechanisms analysis of the reinforcing effect of Epimedii Folium on kidney in the treatment of polycystic ovarian syndrome with network pharmacology

作　　者：张懿丹 麦婷婷 倪喆鑫 丁杰 俞超芹 蔡在龙[3] Zhang Yidan;Mai Tingting;Ni Zhexin;Ding Jie;Yu Chaoqin;Cai Zailong(Student of Traditional Chinese Medicine of 8 Years Program,Basic Medical School,Naval Medical University,Shanghai 200433,China;Gynecology Department of Traditional Chinese Medicine,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Department of Biochemistry and Molecular,Basic Medical School,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学基础医学院,上海200433 [2]海军军医大学附属长海医院中医妇科,上海200433 [3]海军军医大学基础医学院生物化学与分子生物学教研室,上海200433

出　　处：《国际中医中药杂志》2021年第8期796-802,共7页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81573755)。

摘　　要：目的运用网络药理学方法筛选淫羊藿治疗PCOS的有效成分和相关靶点,探讨淫羊藿补肾作用治疗PCOS的作用机制。方法通过检索TCMSP得到淫羊藿的有效成分及其靶点信息;通过NCBI基因数据库将靶点信息转换为基因名称;通过检索GeneCards数据库提取PCOS相关基因;运用韦恩图工具得到淫羊藿治疗PCOS的相关靶点;运用Cytoscape 3.7.2软件构建淫羊藿-有效成分-作用靶点-PCOS网络;通过STRING数据库构建蛋白相互作用网络;并在Geneontology数据库中对相关靶点进行GO富集分析,在KEGG数据库中对相关靶点进行通路富集分析。结果得到23种淫羊藿有效成分及其治疗PCOS的132个相关靶点,淫羊藿补肾作用主要通过调节类固醇激素受体活性等生物学过程及雌激素信号通路、下丘脑促性腺激素释放激素信号通路、下丘脑促性腺激素释放激素分泌、HIF-1信号通路及VEGF信号通路等治疗PCOS。结论本研究从网络药理学角度分析了淫羊藿补肾作用治疗PCOS的相关靶点及信号通路,为后续实验研究和临床运用提供参考。Objective This study by using the method of network pharmacology to screen the active constituent and related targets of Epimedii Folium aims to explore the mechanisms of the reinforcing effect of Epimedii Folium on kidney in the treatment of PCOS.Methods By retrieving data from TCMSP datebase,screened out the active constituent of Epimedii Folium and the information of the targets corresponding to each active constituent;by using the gene database of NCBI,translated the information of the targets into gene names;by retrieving data from GeneCards datebase,extracted the genes related to PCOS;related targets of Epimedii Folium in the treatment of PCOS were obtained by Venn tool;by using Cytoscape 3.7.2 software,constructed a network diagram of Epimedii Folium-active constituents-targets-PCOS;by using STRING database,constructed the protein interaction network;then carried out GO enrichment analysis of related targets by Geneontology database and carried out pathway enrichment analysis of related targets by KEGG database.Results There were 23 active constituents of Epimedii Folium and 132 related targets treating PCOS.The Epimedii Folium could play the reinforcing effect on kidney mainly by regulating the biological processes like steroid hormone receptor activity,as well as KEGG pathways such as Estrogen signaling pathway,GnRH signaling pathway,GnRH secretion,HIF-1 signaling pathway and VEGF signaling pathway in treating PCOS.Conclusion From the perspective of network pharmacology,this study preliminarily analyzed the related targets and pathways of reinforcing effect on kidney of Epimedii folium in the treatment of PCOS,providing reference for further experiments and application inclinics.

关 键 词：淫羊藿 多囊卵巢综合征 补肾 网络药理学 药理作用分子作用机制(中药) 

分 类 号：R285[医药卫生—中药学]