基于蛋白组学探讨丹皮酚和芍药苷配伍抗心肌缺血损伤的作用机制  

Mechanism of paeonol combined with paeoniflorin against myocardial ischemia injury:based on proteomics

作　　者：胡春艳 郭欣[1] 刘雪玲 刘港 许潇[1] 毛庆 郭齐[1] HU Chun-yan;GUO Xin;LIU Xue-ling;LIU Gang;XU Xiao;MAO Qing;GUO Qi(Jiangsu University,Zhenjiang 212013,China;Nanjing Lishui People's Hospital,Nanjing 211200,China)

机构地区：[1]江苏大学,江苏镇江212013 [2]南京市溧水区人民医院,江苏南京211200

出　　处：《中国中药杂志》2021年第15期3943-3948,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81803991);国家中医临床研究基地(江苏省中医院)开放课题(JD2019SZ16);江苏省中医药科技发展计划项目重点项目(ZD201911);江苏省中医药管理局镇江市科技计划项目(SH2018046,SH2018053,SH2019042);2019年度镇江市169人才科研项目;2021年江苏省及江苏大学大学生实践创新训练计划项目。

摘　　要：该研究观察丹皮酚和芍药苷配伍对心肌缺血损伤大鼠心肌蛋白差异表达的影响,探讨二者配伍抗心肌缺血损伤的作用机制。首先通过左冠状动脉前降支结扎术构建急性心肌梗死大鼠模型,用丹皮酚和芍药苷配伍对该模型大鼠进行灌胃给药。然后取大鼠心肌组织提取蛋白样品,运用串联质谱标签(tandem mass tags,TMT)定量蛋白质组学研究药物干预前后的差异蛋白情况。实验结果显示差异蛋白主要富集于phagocytosis engulfment、extracellular space及antigen binding等。KEGG分析显示,主要涉及complement and coagulation cascades、systemic lupus erythematosus及ribosome等信号通路。该研究利用差异蛋白质组学筛选出相关靶蛋白及其所涉及的信号通路可能是丹皮酚和芍药苷配伍抗心肌缺血损伤的作用机制。The study aims to investigate the effect of the compatibility of paeonol and paeoniflorin(hereinafter referred to as the compatibility) on the expression of myocardial proteins in rats with myocardial ischemia injury and explore the underlying mechanism of the compatibility against myocardial ischemia injury. First, the acute myocardial infarction rat model was established by ligation of the anterior descending branch of the left coronary artery. The model rats were given(ig) paeonol and paeoniflorin. Then protein samples were collected from rat cardiac tissue and quantified by tandem mass tags(TMT) to explore the differential proteins after drug intervention. The experimental results showed that differential proteins mainly involved phagocytosis engulfment, extracellular space, and antigen binding, as well as Kyoto encyclopedia of genes and genomes(KEGG) pathways of complement and coagulation cascades, syste-mic lupus erythematosus, and ribosome. In this study, the target proteins and related signaling pathways identified by differential proteomics may be the biological basis of the compatibility against myocardial ischemia injury in rats.

关 键 词：差异蛋白质组学 丹皮酚 芍药苷 心肌缺血损伤 

分 类 号：R285.5[医药卫生—中药学]