甘蔗叶片响应褐锈病菌(Puccinia melanocephala)侵染的转录组分析  

Response of Sugarcane Leaves to the Infection of Puccinia melanocephala: Transcriptomic Analysis

作　　者：高小宁 吴自林 黄咏虹 刘睿 齐永文 Gao Xiaoning;Wu Zilin;Huang Yonghong;Liu Rui;Qi Yongwen(Institute of Bioengineering,Guangdong Academy of Sciences,Guangdong Sugarcane Genetic Improvement Engineering Center,Guangzhou 510316)

机构地区：[1]广东省科学院生物工程研究所/广东省甘蔗遗传改良工程技术中心,广州510316

出　　处：《中国农学通报》2021年第24期102-109,共8页Chinese Agricultural Science Bulletin

基　　金：广东省科学院建设国内一流研究机构行动专项资金项目“甘蔗与褐锈菌互作的细胞学和转录组学分析及抗病相关基因挖掘”(2019GDASYL-0103029);广东省自然科学基金面上项目“自噬相关基因SsATG18a参与甘蔗与褐锈病互作的分子机理研究”(2021A1515011321);广东省甘蔗剑麻产业技术体系创新团队“甘蔗抗性育种岗位”(2019KJ104-05);国家糖料产业技术体系“甘蔗高糖聚合品种改良岗位”(CARS201707)。

摘　　要：旨在从转录水平分析甘蔗叶片响应褐锈病菌侵染的基因表达特征,为进一步研究甘蔗抗褐锈病的分子机理提供基础资料。本研究采用高通量测序技术Illumina Hiseq 2500对健康以及被褐锈病菌侵染的甘蔗叶片进行转录组测序,利用生物信息学方法对差异表达基因进行功能注释和分析,利用实时荧光定量PCR(qRT-PCR)对5个差异基因的相对表达量进行了验证。转录组数据分析共鉴定出差异表达基因4716个,其中上调表达2979个,下调表达1737个。GO注释结果显示,差异表达基因主要聚集在细胞过程、生物过程、催化活性等生理生化过程。KEGG富集分析发现,1919个差异表达基因被注释到114个通路中,其中苯丙烷生物合成、类黄酮生物合成、谷胱甘肽代谢等植物抵御生物胁迫有关的代谢途径的相关基因显著上调表达。qRT-PCR验证5个上调表达基因结果与转录组测序结果的表达趋势一致。The aim is to analyze the gene expression patterns of sugarcane leaves in response to the infection of P.melanocephala,and to provide data for further research on the molecular mechanism of sugarcane resistance to brown rust.The healthy and infected sugarcane leaves by P.melanocephala were sequenced at transcriptomic level using high-throughput sequencing platform,Illumina Hiseq 2500.The functional annotation and analysis for the differentially expressed genes(DEGs)was carried out with bioinformatics methods.The relative expression levels of five DEGs were further verified by real-time fluorescence quantitative PCR(qRT-PCR).The transcriptome data showed that 4716 DEGs were obtained,including 2979 up-regulated and 1737 down-regulated DEGs.GO annotation results showed that DEGs mainly gathered in the physiological and biochemical processes of cell process,biological process,and catalytic activity,etc.KEGG enrichment analysis revealed that 1919 DEGs were annotated into 114 pathways.The relative genes in the metabolic pathways related to plants’resistance to biotic stress were significantly up-regulated,and these metabolic pathways included phenylpropane biosynthesis,flavonoids biosynthesis,and glutathione metabolism and so on.In addition,the qRT-PCR results confirmed that the expression profiles of five up-regulated genes were consistent with that of transcriptome sequencing.

关 键 词：甘蔗 褐锈菌 亲和互作 高通量测序 转录组 差异表达基因 

分 类 号：S432.4[农业科学—植物病理学]