艾纳香内生真菌抗细菌和炭疽菌的活性研究  

Antibacterial and Anti-anthracnose Activities of Endophytic Fungi Colonized in Blumea balsamifera(L.)DC.

作　　者：元超 舒雪纯 张影波 王凯[1] 谢小丽[1] 徐子琪 袁媛 Yuan Chao;Shu Xuechun;Zhang Yingbo;Wang Kai;Xie Xiaoli;Xu Ziqi;Yuan Yuan(Tropical Crops Genetic Resources Institute,CATAS/Hainan Engineering Research Center for Blumea balsamifera,Danzhou Hainan 571737;College of Forestry,Hainan University,Haikou 570228;The First Affiliated Hospital of Shandong First Medical University,Jinan 250014;School of Biosciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/海南省艾纳香工程技术研究中心,海南儋州571737 [2]海南大学林学院,海口570228 [3]山东第一医科大学第一附属医院,济南250014 [4]广东药科大学生命科学与生物制药学院,广州510006

出　　处：《中国农学通报》2021年第23期38-44,共7页Chinese Agricultural Science Bulletin

基　　金：海南省自然科学基金项目“刺盾炱菌Chaetothyriales sp.中新骨架嗜氮酮类化合物的发掘及抗真菌活性”(220RC718);中国热带农业科学院基本科研业务费专项资金“基于微环境互作的艾纳香内生真菌次生代谢产物研究”(1630032019045)。

摘　　要：为获得丰富的艾纳香内生真菌资源,筛选出抗性生防菌株,用于活性次生代谢产物的发现,通过水琼脂法对海南产艾纳香进行内生真菌分离,通过ITS序列分析,对其进行鉴定和分析。采用滤纸片法评价内生真菌发酵产物对细菌的抑制活性,采用平板对峙培养法评价艾纳香内生真菌对植物炭疽菌的拮抗作用。本研究共分离鉴定出191株内生真菌,分属于27属,45种,其中优势菌属为Diaporthe、Paraphoma、Ectophoma、Penicillium、Talaromyces、Hypoxylon、Phomopsis、Colletotrichum和Fusarium。抗细菌活性结果显示,Clonostachys、Fusarium和Diapothe属活性较强。抗炭疽病菌试验显示,Talaromyces、Ectophoma和Penicillium属活性较强。本研究初步探讨了海南产艾纳香内生真菌种类的多样性,并首次对其进行了抗病原菌活性筛查,获得的活性菌株可作为微生物源菌剂开发的重要材料。To obtain abundant endophytic fungi from Blumea balsamifera(L.)DC.,and select bio-control strains for discovery of bioactive metabolites,water-agar method was used to isolate endophytic fungi from Blumea balsamifera(L.)DC distributed in Hainan Island.ITS analysis was adopted for identification and analysis of endophytic fungi.Furthermore,the activities of fermented endophytes culture extract against four strains of bacteria were tested by filtering paper method.Plate confrontation was carried out to evaluate the inhibitory effects of endophytes on four strains of anthracnose.As a result,191 strains were isolated and identified,which belonged to 27 genuses,45 species,the dominant genuses included Diaporthe,Paraphoma,Ectophoma,Penicillium,Talaromyces,Hypoxylon,Phomopsis,Colletotrichum and Fusarium.Clonostachys,Fusarium and Diapothe were more active in antibacterial assay.Anti-anthracnose results showed that the endophyts belonged to Talaromyces,Ectophoma and Penicillium toughly inhibited four strains of anthracnose.In conclusion,our research preliminary discussed the category variety of endophytic fungi derived from Blumea balsamifera collected in Hainan Island,bioactivity-guided screening was carried out firstly,and the selected active endophytic fungi may serve as candidates for founding new agronomic agents.

关 键 词：艾纳香 内生真菌 抗菌活性 抗炭疽菌活性 微生物菌剂 

分 类 号：S482.291[农业科学—农药学]