麋鹿角中核苷类和氨基酸类成分区域差异性分析  

Regional differences of nucleosides and amino acids in Elaphuri Davidiani Cornu

作　　者：王倩[1] 刘睿[1,2] 朱悦 杨滨[3] 丁玉华[4] 白加德[5] 温华军[6] 吴海荣 段金廒 赵明[1] WANG Qian;LIU Rui;ZHU Yue;YANG Bin;DING Yu-hua;BAI Jia-de;WEN Hua-jun;WU Hai-rong;DUAN Jin-ao;ZHAO Ming(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Key Laboratory of Chinese Medicinal Resources Recycling Utilization under National Administration of Traditional Chinese Medicine,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Key Laboratory of Research and Development in Marine Bio-Resource Pharmaceutics,Nanjing 210023,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Dafeng Milu National Nature Reserve,Dafeng 224136,China;Beijing Milu Ecological Research Center,Beijing 100076,China;Shishou Milu National Nature Reserve,Shishou 434407,China;Qinhu Milu National Wetland Park,Taizhou 225500,China)

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心国家中医药管理资源循环利用重点研究室中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023 [2]江苏省海洋药用生物资源研究与开发重点实验室,江苏南京210023 [3]中国中医科学院中药研究所,北京100700 [4]江苏大丰麋鹿国家级自然保护区,江苏大丰224136 [5]北京麋鹿生态实验中心,北京100076 [6]湖北石首麋鹿国家级自然保护区,湖北石首434407 [7]江苏省溱湖国家湿地公园麋鹿苑,江苏泰州225500

出　　处：《中国中药杂志》2021年第14期3494-3503,共10页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2018YFC1706100);江苏省研究生科研创新计划项目(KYCX20_1578)。

摘　　要：为了揭示麋鹿角中核苷类和氨基酸类成分的区域特征,采用UPLC-QTRAPⓇ/MS2方法对4个地区39批次麋鹿角中37种核苷类和氨基酸类成分进行含量测定,并利用正交偏最小二乘判别分析(OPLS-DA)及聚类分析法(CA)进行综合质量评价。结果显示,不同地区麋鹿角中均含有丰富的核苷类和氨基酸类成分,总质量分数分别可达45.09、634.80μg·g-1,所含成分种类及总量差异较小,但各地区之间各成分含量存在显著差异,主要差异成分为Ura、Hpro、Thr、Glu、G5P、2'-dG、Adeno、Met、Ade、Gln、Orni、Phe、2'-dA、Hit、Lys和Ile。其中,Ura、Met、Glu和Ile分别在江苏大丰、江苏溱湖、北京和湖北石首这些区域的麋鹿角药材中含量最高。OPLS-DA及CA分析结果均显示基于核苷类、游离氨基酸类成分的数据信息可将所有麋鹿角样品分为3类,反映出麋鹿角样品的区域特征性。研究结果提示麋鹿角中核苷类、游离氨基酸类成分的积累与其生境密切相关,为麋鹿角药材的品质形成研究、内在质量评价及控制以及麋鹿角的综合利用提供了有益参考。鉴于研究发现,建议可将Ura、Met、Glu和Ile的含量因素作为不同地区药材特征纳入麋鹿角质量标准体系。In order to reveal the regional characteristics of nucleosides and amino acids in Elaphuri Davidiani Cornu,39 samples of Elaphuri Davidiani Cornu collected from 4 different regions were analyzed by UPLC-QTRAPⓇ/MS2 method followed by orthogonal partial least-squares discrimination analysis(OPLS-DA)and cluster analysis(CA).The results showed all the samples contained abundant nucleosides and amino acids,with the total content of 45.09μg·g-1 and 634.80μg·g-1,respectively.The samples presented significant regional differences in the contents of individual components,and the main differential components included Ura,Hpro,Thr,Glu,G5P,2'-dG,Adeno,Met,Ade,Gln,Orni,Phe,2'-dA,Hit,Lys,and Ile.Among them,Ura,Met,Glu,and Ile had the highest content in the samples from Dafeng in Jiangsu,Qinhu in Jiangsu,Beijing,and Shishou in Hubei,respectively.OPLS-DA and CA demonstrated that all the samples of Elaphuri Davidiani Cornu could be divided into three categories,reflecting the regional characteristics.The results indicated that the accumulation of nucleosides and amino acids in Elaphuri Davidiani Cornu was closely related to its habitat,providing a useful reference for the research on the quality formation,quality evaluation and control,as well as the comprehensive utilization of Elaphuri Davidiani Cornu.The findings suggested that the content factors of Ura,Met,Glu,and Ile could be included into the quality standard system of Elaphuri Davidiani Cornu as the characteristics of medicinal materials from different regions.

关 键 词：麋鹿角 核苷类 氨基酸类 区域差异 

分 类 号：R284.1[医药卫生—中药学]