人工老化和自然老化大豆种子的红外光谱研究  

Infrared Spectra of Artificial Aging and Natural Aging of Soybean Seeds

作　　者：刘杰 刘刚[1] 李姝洁 邓子昂 欧全宏[1] 时有明[2] LIU Jie;LIU Gang;LI Shujie;DENG Ziang;OU Quanhong;SHI Youming(School of Physics and Electronic Information, Yunnan Normal University, Kunming 650500, China;School of Physics and Electronic Engineering, Qujing Normal University, Qujing 655011, China)

机构地区：[1]云南师范大学物理与电子信息学院,云南昆明650500 [2]曲靖师范学院物理与电子工程学院,云南曲靖655011

出　　处：《光散射学报》2021年第1期84-92,共9页The Journal of Light Scattering

基　　金：国家自然科学基金项目(31760341);云南省高校科技创新团队支持计划项目资助。

摘　　要：利用傅里叶变换红外光谱、二阶导数红外光谱结合二维相关红外光谱法,研究了人工老化和自然老化条件下的大豆种子。结果显示,两种老化条件下大豆种子的原始光谱整体相似,吸收峰强度出现了差异;在二阶导数红外光谱中,人工老化和自然老化条件下大豆种子中物质变化趋势趋于一致。两种老化大豆种子在1747和1693 cm^(-1)附近的吸光度随着老化程度的增加而增强的趋势;在1660,1549,1236 cm^(-1)附近整体为减弱趋势;在1610~1550 cm^(-1)附近呈现增加趋势;在1150~1100 cm^(-1)附近呈现减弱趋势,但在老化过程中均出现一定增强。二维相关红外光谱结果显示,未经老化处理的大豆种子的自动峰数目和位置相同,自动峰强度发生改变;自然老化条件下大豆种子随着老化程度的加深,自动峰的数量、强度和位置表现出明显差异;人工老化大豆种子随着老化程度的加深,自动峰的数目和位置差异不明显,强度变化较为明显。大豆种子在老化过程中脂类物质增加,蛋白质物质减少,糖类物质减少,酮/醛类物质增加,氨基酸增加,醇类物质、酚类物质发生变化。利用红外光谱法对人工老化和自然老化条件下大豆种子的研究结果表明,红外光谱法可以实现快速、方便的对两种老化大豆种子的光谱变化进行研究。In this paper,soybean seeds under artificial aging and natural aging conditions were studied by infrared spectroscopy.The results showed that the original spectra of soybean seeds under the two aging conditions were similar,and there were differences in the intensity of absorption peaks.In the second derivative infrared spectra,the change trend of substances in soybean seeds under artificial aging and natural aging conditions tends to be consistent.The absorption peak intensity of two kinds of aging soybean seeds increased with the increase of aging degree around 1747 and 1693 cm^(-1).The intensity of absorption peaks around 1660,1549,and 1236 cm^(-1) showed a weakening trend overall.In the vicinity of 1610-1550 cm^(-1),the absorption peak intensity shows an increasing trend.The intensity of the absorption peak near 1150^(-1)100 cm^(-1) shows a weakening trend,but there is a certain increase in the aging process.The results of two-dimensional correlation infrared spectroscopy showed that the number and position of auto-peaks of unaged soybean seeds were the same,and the auto-peaks intensity changed.Under natural aging conditions,the number,intensity and location of auto-peaks of soybean seeds show obvious differences as the degree of aging deepens.As the aging degree of artificially aged soybean seeds deepens,the number and position of auto-peaks are not significantly different,and the intensity changes are more obvious.Lipid substances,amino acids and ketone/aldehyde substances all show an increasing trend during the aging process of soybean seeds,protein substances and sugar substances show a decreasing trend,alcohol substances and phenol substances change.The results of using infrared spectroscopy on soybean seeds under artificial aging and natural aging conditions show that infrared spectroscopy can quickly and conveniently study the spectral changes of two kinds of aging soybean seeds.

关 键 词：大豆种子 人工老化 自然老化 红外光谱 

分 类 号：O436[机械工程—光学工程]