二安替比林甲烷比色法测定家禽饲料和粪便中二氧化钛含量  

Determination of titanium dioxide in poultry feed and feces by diantipyrylmethane colorimetry method

作　　者：王少龙 陈志敏[1] 刘国华[1] 常文环[1] 郑爱娟[1] 蔡辉益[1] Wang Shaolong;Chen Zhimin;Liu Guohua;Chang Wenhuan;Zheng Aijuan;Cai Huiyi(Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]中国农业科学院饲料研究所,北京100081

出　　处：《现代畜牧兽医》2021年第8期9-12,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：现代农业产业技术体系国家肉鸡产业技术体系项目(CARS-42);中国农业科学院科技创新工程协同创新任务“肉鸭高产高效技术集成研究与示范应用”。

摘　　要：为准确评定动物对饲料营养物质的消化能力,研究旨在寻求1种适合在大多数实验室推广、准确度高的二氧化钛含量检测方法。试验样品经强酸消化完全后,加入二安替比林甲烷显色,测定420 nm波长下的吸光度,根据标准曲线计算二氧化钛含量。结果显示,该方法重现性和回收率较好,饲料和粪便的平均加标回收率分别达到100.14%和98.14%。研究表明,二安替比林甲烷比色法操作简便、准确度高,可以用于家禽代谢试验中的二氧化钛含量的测定。In order to accurately assess the digestibility of feed nutrients and the digestibility of feed nutrients by animals,the study aims to find a method for the determination of titanium dioxide content that can be promoted in most laboratories and with high accuracy.After the sample was digested by strong acid digestion,diantibilin methane was added to show the color,the absorbance was determined at 420 nm wavelength,and the titanium dioxide content was calculated according to the standard curve.The results showed that the method had good reproducibility and recovery rate,with the average labeled recovery rate of feed and feces reaching 100.14% and 98.14%,respectively.The study indicates that the diantipyrylmethane colorimetry method is simple and has high accuracy,and it is suitable for the determination of titanium dioxide in poultry metabolic test.

关 键 词：二氧化钛 二安替比林甲烷分光光度法 饲料 粪便 

分 类 号：S816.17[农业科学—饲料科学]