青海不同产地宽叶羌活红外光谱测定分析  

INFRARED SPECTRUM DETERMINATION ANALYSIS OF NOTOPTERYGIUM FORBESII BOISS IN DIFFERENT AREA IN QINGHAI

作　　者：刘国辉[1] 李美雎 朱瑞娟 陈海娟[2] 尚军[2] LIU Guo-hui(Xining Botanical Garden,Xining Qinghai 810008,China)

机构地区：[1]西宁市园林植物园,青海西宁810008 [2]青海师范大学生命科学学院,青海西宁810008

出　　处：《青海草业》2021年第3期14-18,共5页Qinghai Prataculture

基　　金：青海省科技成果转化项目(编号:2018-SF-116);青海省科技成果转化项目(编号:2018-NK-131);青海师范大学中青年科研基金(2017)。

摘　　要：目的:构建青海宽叶羌活(Notopterygium forbesii Boiss)药材的红外光谱,指纹图谱,评价药材质量。方法:以傅里叶变换红外光谱技术测定宽叶羌活在4000~400cm^(-1)范围内的光谱吸收峰,计算二阶导数光谱,并应用主成分分析和系统聚类方法评价羌活化学成分累积量。结果:青海8个产地宽叶羌活有18个红外共有峰,累积化学成分含量大小依次为果洛州军功、玛可河、互助县北山、民和县、贵南县、湟中县、军功帮菜、佑宁寺宽叶羌活;系统聚类分析可以将民和县、果洛军功、玛可河、互助北山、湟中县产宽叶羌活归为一类,帮菜、贵南产宽叶羌活归为一类,佑宁寺产宽叶羌活为一类。结论:该红外光谱方法准确可靠,为宽叶羌活药材鉴定及质量评价提供了参考。Objective: To construct the infrared spectrum and fingerprint of notopterygium macrophyllum in Qinghai Province and evaluate its quality.Methods: the spectral absorption peaks of notopterygium forbesii Boiss in the range of 4 000-400 cm-1 were determined by Fourier transform infrared spectroscopy, and the second derivative spectra were calculated. Principal component analysis and cluster analysis were used to evaluate the accumulation of chemical components in notopterygium forbesii Boiss.Results: there were 18 common infrared peaks of notopterygium macrophyllum from 8 producing areas in Qinghai Province. The order of cumulative chemical components was Guoluo Jungong, Huzhu Beishan, Minhe, Guinan, Huangzhong, Bangcai, Youningsi and Makehe;The results of cluster analysis showed that notopterygium macrophyllum from Minhe, Guoluo military industry, Huzhu Beishan, Makehe and Huangzhong could be classified into one group, while notopterygium macrophyllum from Bangcai and Guinan could be classified into one group, and notopterygium macrophyllum from Youning temple could be classified into one group.Conclusion: the method is accurate and reliable, which provides a reference for the identification and quality evaluation of notopterygium macrophyllum.

关 键 词：红外光谱 宽叶羌活 

分 类 号：S812.8[农业科学—草业科学]