铁皮石斛的组织培养和快速无性繁殖  

STUDY ON RAPID PROPAGATION SYSTEM OF DENDROBIUM OFFICINALE

作　　者：姜燕燕 李建民 JIANG Yan-yan(Plant Tissue Culture Laboratory College of Life Sciences,Qinghai Normal University,Xining Qinghai 810008,China)

机构地区：[1]青海师范大学生命科学学院植物组织培养实验室,青海西宁810008

出　　处：《青海草业》2021年第3期19-25,共7页Qinghai Prataculture

摘　　要：为了提高铁皮石斛在快速繁殖中的繁殖系数,缩短育苗的周期。以铁皮石斛的带节茎段为外植体,以MS培养基为基本培养基,采用正交实验的设计方法筛选出铁皮石斛在不定芽的诱导和不定芽增殖过程中6-BA,NAA,GA的最佳浓度配比,还研究了不同浓度NAA和IBA对铁皮石斛生根的影响。结果表明;不定芽诱导最佳的激素浓度配比为2 mg/L+6-BA 0.2 mg/LNAA+0.2 mg/LGA,不定芽增殖最佳的激素浓度配比为1 mg/L6-BA+0.5 mg/LNAA+0.2 mg/LGA,只添加NAA对不定芽生根有促进作用,当NAA浓度为0.3 mg/L时,不定芽生根效果最好。In order to study the effects of different hormones and different concentrations of different hormones on the growth rate adventitious bud induction, cluster seedling increment and cluster seedling rooting of Dendrobium officinale in tissue culture and rapid propagation process. Dendrobium officinale shoot segment with buds was used as explant, MS as basic medium, and different kinds and concentrations of hormones were added to the medium. The growth rate and condition of Dendrobium officinale were observed regularly, and the most suitable medium for each stage was selected. The results showed that the most suitable medium for bud induction was: MS+0.2 mg/LNAA+2 mg/L6-BA,The most suitable medium for multiplication of cluster buds was: MS+0.5 mg/L+1 mg/L6-BA. The optimum medium for seedling rooting is MS+0.3 mg/LNAA.

关 键 词：铁皮石斛 快速繁殖 不定芽的诱导 增殖 生根 

分 类 号：S339.41[农业科学—作物遗传育种]