灯盏花素对创伤性损伤大鼠的神经保护作用  

Neuroprotective effect of breviscapine in rats with traumatic brain injury

作　　者：刘维[1] 贺承健[1] 张剑锋[1] LIU Wei;HE Cheng-jian;ZHANG Jian-feng(Intensive Care Unit of the Affiliated Nanhua Hospital,University of South China,Hengyang,Hunan Province 421002,China)

机构地区：[1]南华大学附属南华医院重症医学科,湖南衡阳421002

出　　处：《中国公共卫生》2021年第7期1129-1133,共5页Chinese Journal of Public Health

基　　金：湖南省卫生计生委2017年度科研计划(B2017061)。

摘　　要：目的探讨灯盏花素(Bre)基于活性氧簇(ROS)-NOD样受体蛋白3(NLRP3)炎症小体通路对创伤性脑损伤(TBI)大鼠的神经保护作用。方法构建TBI大鼠模型,实验分为假手术组、模型组、低、中、高剂量Bre组、胞磷胆碱钠组。采用神经功能缺损评分表(NSS)对大鼠神经功能评分;测定脑组织含水量和血脑屏障通透性;酶联免疫吸附(ELISA)法测定脑组织中S100B蛋白(S100B)、神经元特异性烯醇化酶(NSE)、肿瘤坏死因子–α(TNF-α)、白细胞介素1β(IL-1β)、IL-18、IL-6水平;试剂盒测定超氧化物歧化酶(SOD)、丙二醛(MDA)、脑组织中ROS水平;蛋白印迹法测定脑组织中NLRP3、caspase-1水平。结果与假手术组比较,TBI组大鼠NSS评分、脑组织含水量、EB含量和MDA、S100B、NSE、TNF-α、IL-1β、IL-8、IL-6、ROS、NLRP3、caspase-1水平显着升高(P<0.05),SOD水平明显降低(P<0.05);大鼠海马区锥体细胞排列散乱,细胞缩小、核仁不明显。与TBI组比较,大鼠NSS评分、脑组织含水量、EB含量和MDA、S100B、NSE、TNF-α、IL-1β、IL-8、IL-6、ROS、NLRP3、caspase-1水平明显降低(P<0.05),SOD水平明显升高(P<0.05),大鼠海马区锥体细胞结构层次分明,排列恢复紧密,坏死细胞数减少。结论Bre可能通过下调TNF-α、IL-1β、IL-8、IL-6、MDA水平,上调SOD水平以缓解TBI大鼠症状,其机制可能与ROS-NLRP3信号通路有关。Objective To explore neuroprotective effect of breviscapine(Bre)and its mechanism in rats with traumatic brain injury(TBI).Methods TBI model was established in specific pathogen free Wistar rats and the rats were divided into 6 groups(24 in each group):a sham operation group and a TBI model group with saline,three dose groups with low,moderate and high Bre(6,12 and 24 mg/kg),and a citicoline sodium(100 mg/kg)group;all the treatments were administered intragastrically once a day continuously for 5 days.Neurological severity score(NSS)was used to evaluate the neurological function of the rats at 5th day of the treatments.Water content of brain tissues and the permeability of blood-brain barrier were determined and brain tissue samples were collected for all the rats by the end of the treatments.The levels of S100B protein(S100B),neuron-specific enolase(NSE),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-18(IL18)and interleukin-6(IL-6)were measured with enzyme-linked immunosorbent assay(ELISA);the levels of superoxide dismutase(SOD),malondialdehyde(MDA),and reactive oxygen species(ROS)were detected with kit assay;and the levels of NOD-like receptor protein 3(NLRP3)and caspase-1 were detected with Western blot.Results Compared with those in the rats with sham operation,the NSS score,brain water content,content of evansblue(EB),and levels of MDA,S100B,NSE,TNF-α,IL-1β,IL-8,IL-6,ROS,NLRP3,caspase-1 increased significantly(all P<0.05)but the level of SOD decreased significantly(P<0.05)in the TBI model rats.The pyramidal cells with scattered arrangement,shrunken cell bodies and indistinct nucleoli were observed in hippocampal regions of TBI model rats.In comparison with those in the TBI model rats,the NSS score,brain water content,content of EB and levels of MDA,S100B,NSE,TNF-α,IL-1β,IL-8,IL-6,ROS,NLRP3,and caspase-1 decreased significantly(all P<0.05)but the level of SOD increased significantly(P<0.05)in the TBI model rats with Bre treatments;the pyramidal cells with better arrangement and cell

关 键 词：灯盏花素 活性氧簇(ROS) NOD样受体蛋白3(NLRP3) 创伤性脑损伤(TBI) 

分 类 号：R338.1[医药卫生—人体生理学]