刺五加化学成分的分离与鉴定  

Isolation and identification of chemical constituents from Acanthopanax senticosus

作　　者：粟杰 冯艳 林炳锋 李艳[1] 黄文平 王琦[1,2] SU Jie;FENG Yan;LIN Bing-feng;LI Yan;HUANG Wen-ping;WANG Qi(National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment,Nanchang 330006,China)

机构地区：[1]江西中医药大学中药固体制剂制造技术国家工程研究中心,江西南昌330006 [2]创新药物与高效节能降耗制药装备国家企业重点实验室,江西南昌330006

出　　处：《中草药》2021年第16期4783-4788,共6页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划(2019YFC1712302);江西省自然科学基金面上项目重点项目(20171ACB20039);南昌市创新人才团队([2018]274);南昌市中药活性成分与天然药物重点实验室(2019-NCZDSY-011)。

摘　　要：目的对刺五加Acanthopanax senticosus的化学成分进行研究。方法采用Sephadex LH-20、硅胶、半制备高效液相和其他色谱技术分离和纯化化学成分,通过其理化性质和NMR数据鉴定化合物的结构。结果从60%乙醇提取物中分离得到21个化合物,分别鉴定为异落叶松脂醇(1)、dihydrodehydrodiconiferyl alcohol(2)、苦楝新醇(3)、vanillil(4)、2,3-二氯喹喔啉(5)、1,5-二咖啡酰奎宁酸(6)、原儿茶醛(7)、3-carboxy-6,7-dihydroxy-1-(3’,4’-dihydroxyphenyl)-naphthalene(8)、咖啡酸乙酯(9)、5,6,7-三甲氧基香豆素(10)、大黄素-1-O-β-D-葡萄糖苷(11)、对香豆酸(12)、秦皮乙素(13)、methyl 3,5-dihydroxy-4-methoxybenzoate(14)、异嗪皮啶(15)、(-)-丁香树脂酚(16)、(+)-丁香脂素(17)、反式咖啡酸(18)、大黄素(19)、香草酸(20)、咖啡酸甲酯(21)。结论化合物1、3~5、7~11、13、14为首次从刺五加属植物中分离得到。Objective To study the chemical constituents from Ciwujia(Acanthopanax senticosus).Methods The chemical constituents were separated and purified by Sephadex LH-20,silica gel,semi-prepared HPLC,and other chromatography techniques.Their structures were elucidated by their physicochemical properties and NMR data.Results Twenty-one compounds were isolated from the 60%ethanol extract and identified as(+)-isolariciresinol(1),dihydrodehydrodiconiferyl alcohol(2),melianoninol(3),vanillil(4),2,3-dichloroquinoxaline(5),1,5-di-O-caffeoylquinic acid(6),protocatechuylaldehyde(7),3-carboxy-6,7-dihydroxy-1-(3′,4′-dihydroxyphenyl)-naphthalene(8),ethylcaffeate(9),5,6,7-trimethoxycoumarin(10),emodin-1-O-β-D-glucopyranoside(11),p-coumaric acid(12),aesculetin(13),methyl 3,5-dihydroxy-4-methoxybenzoate(14),isofraxidin(15),(-)-syringaresinol(16),(+)-syringaresinol(17),trans-caffeicacid(18),emodin(19),vanillic acid(20)and methyl caffeate(21).Conclusion Compounds 1,3—5,7—11,13,14 are isolated from the genus of Acanthopanax for the first time.

关 键 词：刺五加 苦楝新醇 原儿茶醛 异嗪皮啶 大黄素 香草酸 

分 类 号：R284.1[医药卫生—中药学]