pH值和表面活性剂对桦褐孔菌降解甘蔗渣效率的影响  

Effects of pH value and surfactant on degradation efficiency of sugarcane bagasse by Inonotus obliquus

作　　者：张超[1] 王天珍 陈肖肖 徐向群[1] ZHANG Chao;WANG Tianzhen;CHEN Xiaoxiao;XU Xiangqun(College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China)

机构地区：[1]浙江理工大学生命科学与医药学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2021年第5期704-710,共7页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省自然科学基金项目(LY16B020013)。

摘　　要：为了提高桦褐孔菌(Inonotus obliquus)液体发酵选择性降解甘蔗渣木质素的效率,采用两因素多水平的方法进行正交实验,并通过正交实验确定发酵条件参数,对不同发酵时间处理后的甘蔗渣进行糖化水解,分析pH值和表面活性剂(Tween-80)对桦褐孔菌降解甘蔗渣的影响。结果表明:当pH值为6.0,Tween-80添加量为0.1%时,桦褐孔菌表现出较强的选择性降解效率,最高木质素降解率由对照的21.3%提升到76.6%,并且达到最高木质素降解率的发酵时间由10 d缩短至2 d,最高产糖量和糖化效率也分别由对照的188.0 mg/g、22.8%提升至246.4 mg/g、30.5%;pH值6.0和0.1%Tween-80对桦褐孔菌选择性降解甘蔗渣木质素有较好的促进作用。In order to improve the efficiency of selective degradation of sugarcane bagasse lignin by Inonotus obliquus in liquid fermentation,we carried out an orthogonal experiment in a two-factor multi-level manner,the fermentation parameters were determined through the orthogonal experiment,the bagasse treated with different fermentation times was saccharified and hydrolyzed,and the effects of pH value and surfactant(Tween-80)on the degradation of bagasse by Inonotus obliquus were evaluated.The results showed that when pH value was 6.0 and the addition of Tween-80 was 0.1%,Inonotus obliquus showed strong selective degradation efficiency,the maximum lignin degradation rate increased from 21.3%for the control group to 76.6%,and the fermentation time to achieve the maximum lignin degradation rate was shortened from 10 d to 2 d.The maximum sugar yield and saccharification efficiency also grew from 188.0 mg/g and 22.8%to 246.2 mg/g and 30.5%.It was proved that proper pH value and addition of Tween-80(6.0 and 0.1%,respectively)can improve the selective degradation of sugarcane bagasse lignin by Inonotus obliquus and significantly promote the saccharification of bagasse.

关 键 词：桦褐孔菌 液体发酵 选择性降解 PH值 TWEEN-80 糖化作用 

分 类 号：Q933[生物学—微生物学]