2019年濮阳市登革热疫情与病原分子溯源  

The first outbreak of dengue fever and molecular tracing in Puyang, 2019

作　　者：徐国锋[1] 张清杰[1] 张玉华[2] 孟晓飞[1] 李广康 郭永豪[3] 谢为民[2] 夏胜利[4] Xu Guofeng;Zhang Qingjie;Zhang Yuhua;Meng Xiaofei;Li Guangkang;Guo Yonghao;Xie Weimin;Xia Shengli(Microbiological Laboratory of Puyang Center for Disease control and prevention,Puyang 457000,China;Puyang Center for Disease control and prevention,Puyang 457000,China;Institute for Immunization Programme,Henan Center for Disease Control and Prevention,Zhengzhou 450016,China;Institute for Infectious Disease Control and Prevention,Henan Center for Disease Control and Prevention,Zhengzhou 450016,China)

机构地区：[1]濮阳市疾病预防控制中心微生物检验科,457000 [2]濮阳市疾病预防控制中心,457000 [3]河南省疾病预防控制中心免疫规划所,郑州450016 [4]河南省疾病预防控制中心传染病预防控制所,郑州450016

出　　处：《中华预防医学杂志》2021年第8期978-982,共5页Chinese Journal of Preventive Medicine

摘　　要：目的分析河南省濮阳市2019年8月份的一起登革热暴发疫情。方法利用胶体金法检测登革病毒IgM/IgG抗体和NS1抗原,利用PCR方法扩增E基因,并进行基因测序,利用MegaX软件进行E基因编码蛋白序列的比对分析,构建进化树。结果经过临床确诊和实验室确诊,共有81例登革热病例,其中17例为当地农民到柬埔寨和泰国务工的输入性病例,64例为本土病例。E基因编码的蛋白序列比对分析结果表明,该起疫情的病原为登革热病毒1型,与越南株高度同源。本土疫情发生后,登革病毒E基因发生了核苷酸位点突变,突变位点可稳定传播。结论濮阳本起登革热暴发疫情系与国外输入病例相关联的一起由登革1型病毒引起的本地暴发,基因测序显示输入性病原在本地流行后发生了较为稳定且可传播的核苷酸突变。Objective This study retrospectively analyzed an outbreak of dengue fever in Puyang of Henan province in 2019,in order to find the sources of infection.Methods Dengue virus IgM/IgG and NS1 antigen were tested by colloidal gold method.E gene was amplified by PCR.MegaX was used for sequences alignment to construct evolutionary distance trees.Results After clinical and laboratory confirmation,there were 81 cases of dengue fever,17 of which were imported case who were local farmers and worked in Combadia and Thailand,and 64 of which were indigenous cases.The E gene alignment results showed that the pathogen of this epidemic was Vietnamese 1 and highly homologous with the Vietnamese strain.After the local outbreak,dengue virus E gene developed a nucleotide site mutation which can be steadily transmission.Conclusion The dengue fever outbreak in Puyang was a local outbreak caused by dengue virus type 1,which was associated with imported cases.Gene sequencing showed that the imported pathogen had a relatively stable and transmissible nucleotide mutation after the local epidemic.

关 键 词：登革热病毒 基因型 诊断 分子溯源 

分 类 号：R512.8[医药卫生—内科学] R181.3[医药卫生—临床医学]