2015-2020年河南省患者来源单核细胞增生李斯特菌流行特征分析  

Analysis on epidemiological characteristics of Listeriamonocytogenes in patients from Henan province between 2015 to 2020

作　　者：炊慧霞[1] 戚浩彧 邱正勇[1] 李薇薇 贾松树[1] 王干一 张秀丽[1] 廖兴广[1] 韩志伟 Chui Huixia;Qi Haoyu;Qiu Zhengyong;Li Weiwei;Jia Songshu;Wang Ganyi;Zhang Xiuli;Liao Xingguang;Han Zhiwei(Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016,China;China National Center for Food Safety Risk Assessment,Beijing 100022,China)

机构地区：[1]河南省疾病预防控制中心,郑州450016 [2]国家食品安全风险评估中心,北京100022

出　　处：《中华检验医学杂志》2021年第8期749-754,共6页Chinese Journal of Laboratory Medicine

基　　金：“十三五”国家重点研发计划(2017YFC1601501);河南省医学科技攻关计划省部共建项目(SBGJ2018091)。

摘　　要：目的分析河南省患者来源单核细胞增生李斯特菌的血清学和分子分型,了解河南省李斯特菌病流行情况,构建患者分离株分子溯源数据库,为李斯特菌病溯源提供实验室依据。方法参照国家食源性疾病监测工作手册,2015年1月至2020年7月河南省单核细胞增生李斯特菌感染病例专项监测从16家哨点医院监测李斯特菌病阳性病例71例,采集阳性病例标本80份进行检测,对获得的阳性菌株71株进行分子分型,参照中华人民共和国出入境检验检疫行业标准单核细胞增生李斯特菌血清分型方法(SN/T 2521-2010)和单核细胞增生李斯特菌诊断血清使用说明书对获得的80株单核细胞增生李斯特菌进行血清学分型,参照国家食源性疾病分子溯源网络使用手册进行聚丙烯酰胺凝胶电泳(PFGE)聚类分析。结果共监测到71例李斯特菌病阳性病例,其中38例为围产期病例,33例为非围产期病例。80份李斯特菌病阳性病例标本58.75%(47/80)来自围产期病例,20.00%(16/80)来自非围产期有基础病病例;来自于非围产期年龄>1个月~≤5岁、>5~≤60岁和>60岁人群分别为7.50%(6/80)、12.50%(10/80)和1.25%(1/80)。标本类型分为5类,73.75%(59/80)为血液,15.00%(12/80)为脑脊液,粪便、宫腔拭子、痰液各占3.75%(3/80)。对获得的80株单核细胞增生李斯特菌进行血清学分型,分属3个血清型,1/2b型、1/2a型和4b型分别占61.25%(49/80)、35.00%(28/80)和3.75%(3/80);71株单核细胞增生李斯特菌经AscⅠ酶切,获得58种带型,每种带型包括1~4株菌株,相似度为60.8%~100%。GX6A16HA0005、GX6A16HA0011、GX6A16HA0030、GX6A16HA0023、GX6A16HA0029和GX6A16HA0054为优势带型,依次包括4、4、4、3、2和2株菌;GX6A16HA0005带型包括的4株菌分离自2016、2018和2020年,其中2016年(1株)和2018年(1株)均来自濮阳市;GX6A16HA0011带型包括的4株分离自2016、2018和2020年,其中2020年2株均来自洛阳市;GX6A16HA0030带型包括的4株均分�Objective To analyze the serology and molecular typing of Listeriamonocytogenes isolated from patients in Henan,and to explore the epidemic situation of listeriosis,construct the molecular traceability database of patient isolates,so as to provide laboratory basis for listeriosis traceability.Methods From January 2015 to July 2020,71 positive Listeriamonocytogenes cases were monitored in 16 sentinel hospitals in Henan.Eighty samples of positive cases were collected for detection,and 71 positive strains were obtained for molecular typing.According to the Entry-exit Inspection and Quarantine Industry Standard of China(SN/T 2521-2010)and the instructions for the use of diagnostic serum of Listeriamonocytogenes,80 strains of Listeriamonocytogenes were serotyped,and PFGE cluster analysis was performed according to the User Manual of National Foodborne Disease Molecular Traceability Network.Results A total of 71 positive listeriosis cases were detected,of which 38 cases were perinatal cases and 33 cases were non-perinatal cases.Among the 80 positive cases of listeriosis,58.75%(47/80)were from perinatal cases,20.00%(16/80)were from non-perinatal cases with underlying diseases,the proportion of>1 month-≤5 years old,>5-≤60 years old and>60 years old was 7.50%(6/80),12.50%(10/80)and 1.25%(1/80),respectively,in non-perinatal age group.There were 5 types of specimens,73.75%(59/80)were blood,15.00%(12/80)were cerebrospinal fluid,and 3.75%(3/80)were stool,intrauterine swab and sputum.80 strains of Listeriamonocytogenes were classified into three serotypes,Type 1/2b,Type 1/2a and Type 4b accounted for 61.25%(49/80),35.00%(28/80)and 3.75%(3/80)respectively.The 71 strains of Listeriamonocytogenes were digested by AscⅠ,and 58 bands were obtained.Each band type included 1-4 strains,and the similarity was 60.8%-100%.GX6A16HA0005,GX6A16HA0011,GX6A16HA0030,GX6A16HA0023,GX6A16HA0029 and GX6A16HA0054 were dominant bands,including 4,4,4,3,2 and 2 strains respectively.Four strains of GX6A16HA0005 from 2016,2018 and 2020 were isolate

关 键 词：李斯特菌病 血清学 分子分型 流行特征 

分 类 号：R446.5[医药卫生—诊断学]