基质刚度调节细胞-细胞外基质间黏附对肿瘤细胞迁移影响的模型研究  

Influences of Cell-ECM Adhesion on Migration of Tumor Cells Regulated by ECM Stiffness: A Model Study

作　　者：张颖 王钰岚 王楷群 魏延 黄棣 陈维毅[1,2] 单彦虎 ZHANG Ying;WANG Yulan;WANG Kaiqun;WEI Yan;HUANG Di;CHEN Weiyi;SHAN Yanhu(Research Center for Nano-Biomaterials&Regenerative Medicine,College of Biomedical Engineering,Department of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China;Shanxi Key Laboratory of Material Strength&Structural Impact,Institute of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China;School of Instrument and Electronics,North University of China,Taiyuan 030051,China)

机构地区：[1]太原理工大学生物医学工程学院生物医学工程系,纳米生物材料与再生医学研究中心,太原030024 [2]太原理工大学生物医学工程研究所,山西材料强度与结构冲击重点实验室,太原030024 [3]中北大学仪器电子学院,太原030051

出　　处：《医用生物力学》2021年第4期604-611,共8页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(11502156,11632013,11502158,51503140)。

摘　　要：目的研究细胞外基质(extracellular matrix,ECM)刚度对细胞和ECM间黏附及肿瘤迁移的影响。方法建立基于网状波茨模型(cellular Potts model,CPM),模拟肿瘤细胞生长与细胞间免疫反馈过程,观察细胞力学行为改变对细胞-ECM黏附的影响状况,分析不同ECM下肿瘤细胞迁移的变化。结果ECM刚度变化会影响肿瘤细胞的迁移速度。ECM刚度改变调节细胞与ECM的黏附力,黏附力改变影响细胞的迁移速度。结论细胞的迁移和分布模式与ECM黏附性以及刚度密切相关。基质刚度增加促进肿瘤细胞在较低刚度下迁移,而基质刚度进一步增加抑制肿瘤细胞迁移。研究结果可进一步揭示ECM动态变化、黏附大小及肿瘤细胞迁移的力学表现。Objective To study the influence of cell-extracellular matrix(ECM)adhesion on migration of tumor cells regulated by ECM stiffness.Methods The cellular Potts model(CPM)was established to simulate tumor cell growth and cellular immune feedback system.The effects from mechanical behavior of cells on cell-ECM adhesion were observed,and the migration of tumor cells under different ECM was analyzed.Results The ECM stiffness could influence the migration rate of tumor cells.The change of ECM stiffness regulated the adhesion force between cells and ECM,and the change of adhesion force would influence the migration rate of cells.Conclusions The migration and distribution patterns of cells are closely related to the adhesion and stiffness of ECM.The increase in ECM stiffness can effectively promote the migration rate of tumor cells,and the further increase in ECM stiffness inhibits the migration of tumor cells.These findings may further reveal dynamic changes of ECM,adhesion and mechanical performance of tumor cell migration.

关 键 词：肿瘤细胞 细胞生长 细胞迁移 细胞黏附 细胞外基质 网状波茨模型 

分 类 号：R318.01[医药卫生—生物医学工程]