BPX-80离心式血泵中剪切力引发血管性血友病因子机械损伤的评价  

Evaluation on Mechanical Damage of vWF Induced by Shear Stress in BPX-80 Centrifugal Blood Pump

作　　者：钟敏 葛婉宁 张柳笛 梅旭 ZHONG Min;GE Wanning;ZHANG Liudi;MEI Xu(Artificial Organ Technology Laboratory,Bio-Manufacturing Research Centre,School of Mechanical and Electrical Engineering,Soochow University,Suzhou 215006,Jiangsu,China)

机构地区：[1]苏州大学机电工程学院,生物制造研究中心,人工器官研究所,江苏苏州215006

出　　处：《医用生物力学》2021年第4期646-651,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(31700817);江苏省自然科学基金项目(BK20170352);江苏省高校自然科学研究面上项目(17KJB416010);第62批国家博士后基金(2017M620224)。

摘　　要：目的对BPX-80离心式血泵中剪切力引起血管性血友病因子(von Willebrand Factor,v WF)的损伤进行评价,判断其是否可以作为v WF损伤研究的参照泵。方法按照ASTM标准搭建体外溶血测试平台,使用新鲜猪血在此平台对BPX-80离心式血泵进行8 h测试,然后评估每小时血液样本的溶血水平和v WF损伤,并与静态对照组进行比较。结果在整个实验过程中,BPX-80的溶血指数保持稳定且较低;高分子量v WF多聚体有少量降解,与静置对照组相比无显著性差异;v WF抗原含量无明显变化,与静置对照组趋势基本一致。结论BPX-80离心式血泵血液相容性良好,可以作为v WF损伤以及溶血评价实验的基准参照泵,从而为新型血泵的设计与优化提供参考。Objective To evaluate the damage of von Willebrand factor(vWF)induced by shear stress in BPX-80 centrifugal blood pump,and determine whether it can be used as a reference pump for v WF damage research.Methods An in vitro hemolysis test platform was established according to the ASTM standards.The BPX-80 centrifugal blood pump was tested for 8 hours by using fresh porcine blood.The hemolysis level and v WF damage of hourly blood samples were then evaluated and compared with the static control group.Results The hemolysis index of BPX-80 was stable and low during the test;v WF polymer with high molecular weight had a small amount of degradation,and showed no significant difference compared with the static control group;there was no significant change in the concentration of vWF antigen,which was basically consistent with the trend of the static control group.Conclusions BPX-80 centrifugal blood pump has good blood compatibility and can be used as the reference pump for vWF damage and hemolysis evaluation,thereby providing guidance for the design and optimization of new blood pumps.

关 键 词：心室辅助装置 血管性血友病因子 血液损伤 非生理性剪切力 

分 类 号：R318.01[医药卫生—生物医学工程]