楸树实生苗和嫁接苗根相关真菌群落结构和多样性  

Community structure and diversity of root-associated fungi of Catalpa bungei seedlings and grafted seedlings

作　　者：蒙盼盼 冯欢[1] 陈伟[1] 王海华 汤夏安 陈娟 辛珂绪 牟雪丽 王春燕[1] MENG Pan-Pan;FENG Huan;CHEN Wei;WANG Hai-Hua;TANG Xia-An;CHEN Juan;XIN Ke-Xu;MOU Xue-Li;WANG Chun-Yan(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China;North Florida Research and Education Center,Soil and Water Science Department,University of Florida,155 Research Road,Quincy,FL 32351,USA)

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]North Florida Research and Education Center,Soil and Water Science Department,University of Florida,155 Research Road,Quincy,FL 32351,USA

出　　处：《菌物学报》2021年第8期1965-1979,共15页Mycosystema

基　　金：国家重点研发计划(2017YFD0600604);中央高校基本科研业务费专项(2452019059)。

摘　　要：以1、2、3年生的楸树实生苗和嫁接苗(梓树砧木)根系为研究对象,通过对ITS rDNA区域标记扩增子的Illumina MiSeq测序,分析不同苗龄楸树实生苗和嫁接苗根相关真菌的结构组成和多样性。获得根相关真菌OTU共842个,分属4门、24纲、70目、134科、233属、347种;丛枝菌根真菌(arbuscular mycorrhizal fungi)AMF-OTU共42个,分属1门、1纲、3目、3科、3属、13种。根相关真菌和AMF的OTU数量、丰度和多样性在实生苗中均随苗龄的增加而降低,而在嫁接苗中则随着苗龄的增加而增加。门水平上,实生苗与嫁接苗根相关真菌的优势菌都是子囊菌门Ascomycota、担子菌门Basidiomycota和接合菌门Zygomycota,但它们的相对丰度有所差异;属水平上,实生苗和嫁接苗根相关真菌的优势菌种在组成和数量上都具有一定的差异性。楸树根相关真菌拥有3种营养模式和12个生态功能群,其中实生苗根系中病理营养型真菌的比例大于嫁接苗,腐生营养型则差异不大,而共生营养型则小于嫁接苗。生态功能群分析显示大多数楸树根系真菌表现出多种生存策略,部分真菌可以在植物-真菌-动物中跨界侵染。该研究可为楸树根相关真菌的利用提供一定的理论依据和基础。The community structure and diversity of root-associated fungi of roots of 1,2,3-year-old Catalpa bungei seedlings and grafted seedling(C.ovata as rootstock)at different seedling ages were analyzed by Illumina MiSeq sequencing of ITS rDNA region marker amplicons.A total of 842 root-associated fungi OTUs was obtained,belonging to 4 phyla,24 classes,70 orders,134 families,233 genera and 347 species,including 42 arbuscular mycorrhizal fungal(AMF)OTUs belonging to 1 phyla,1 class,3 orders,3 families,3 genera and 13 species.The OTU number,abundance and diversity of root-associated fungi and AMF decreased with the increase of seedling age,but increased with the increase of grafted seedling age.At the phylum level,Ascomycota,Basidiomycota and Zygomycota were dominant,but their respective relative abundances were different.At the genus level,the root associated dominant fungi differed in composition and number of species.The root-associated fungi of C.bungei had three nutritional modes and 12 ecological functional groups.The proportion of pathotrophic fungi in the roots of seedlings was higher than that in the roots of grafted seedlings,but the proportion of saprotrophic fungi had little difference,while the symbiotrophic fungi in roots of seedlings were less than those in roots of grafted seedlings.The analysis of ecological functional groups showed that most of the root-associated fungi of C.bungei exhibited a variety of survival strategies,and some of them were transkingdom(plants-fungi-animals)parasites.

关 键 词：楸树 根相关真菌 可操作分类单元 丰度 生态功能群 

分 类 号：S792.99[农业科学—林木遗传育种]