混合接种球孢白僵菌与摩西球囊霉对烟草促生抗逆影响  

Effects of mixed inoculation of Beauveria bassiana and Glomus mosseae on tobacco growth and stress resistance

作　　者：许绍欢 许忠顺 杜飞 刘京 芶剑渝 邹晓[1] XU Shao-Huan;XU Zhong-Shun;DU Fei;LIU Jing;GOU Jian-Yu;ZOU Xiao(Institute of Fungus Resources,Department of Ecology,College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou University,Guiyang,Guizhou 550025,China;Guizhou Tobacco Company Zunyi Branch,Zunyi,Guizhou 563000,China)

机构地区：[1]贵州大学生命科学学院生态系真菌资源研究所,贵州贵阳550025 [2]贵州大学山地植物资源保护与种质创新省部共建教育部重点实验室,贵州贵阳550025 [3]贵州省烟草公司遵义市公司,贵州遵义563000

出　　处：《菌物学报》2021年第8期2191-2200,共10页Mycosystema

基　　金：贵州省烟草公司遵义市公司科技项目(2018-02);贵州中烟工业有限责任公司科技项目(GZZYKJJS2016BY020-1)。

摘　　要：利用球孢白僵菌Beauveria bassiana与摩西球囊霉菌Glomus mosseae以单独和混合接种的方式在烟草Nicotiana tabacum苗期进行接种处理,检测烟草苗期和成熟期的农艺性状和生理特性。结果表明:在育苗期,混合接种处理烟苗具有最大的叶长和最大叶宽,与对照相比提高了30.27%和42.98%,球孢白僵菌处理组生物量最大,AM真菌处理组烟草株高最高;在成熟期,混合接种处理组烟草的株高、最大叶长、最大叶宽、茎围、生物量等指标均优于单接种处理,且过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)等抗氧化酶活性最高,丙二醛(MDA)含量最低。球孢白僵菌和AM真菌可协同提高烟草生长和抗逆能力。Tobacco(Nicotiana tabacum)seedlings were inoculated with Beauveria bassiana,Glomus mosseae and B.bassiana+G.mosseae,and the agronomic and physiological characteristics of the plants in seedling and maturity stages were evaluated.The results showed that in the seedling stage,the leaf length and leaf width of the tobacco were maximal,increasing 30.27%and 42.98%respectively as against experimental control after mixed inoculation treatment.The plant biomass of B.bassiana treatment group was the largest,and the G.mosseae treatment group gained the tallest plant height.At the maturity stage,the plant height,maximum leaf length,maximum leaf width,stem circumference,biomass and other indicators of tobacco in the mixed inoculation treatment group were superior to those in the separate inoculation treatment group.Meanwhile,the activities of catalase(CAT),superoxide dismutase(SOD),peroxidase(POD)and other antioxidant enzymes were the highest and the content of malondialdehyde(MDA)was the lowest in mixed inoculation treatment group.In conclusion,B.bassiana and G.mosseae can synergistically improve the growth and stress resistance ability of tobacco.

关 键 词：AM 真菌 球孢白僵菌 烟草 生物量 抗氧化酶 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]