雷公藤多苷片治疗关节炎机制的网络药理学研究  

Mechanism of tripterygium glycoside tablets in treating rheumatoid arthritis based on network pharmacology

作　　者：刘盼盼[1] 郭盈盈[2] LIU Panpan;GUO Yingying(Bone and Joint Sports Bone and Soft Tissue Tumor Ward,Bone Surgery,Shengjing Hospital,China Medical University,Shenyang 110016,China;Rheumatoid Immunization Ward,Internal Medicine,Shengjing Hospital,China Medical University,Shengyang 110016,China)

机构地区：[1]中国医科大学附属盛京医院骨外科骨关节运动骨与软组织肿瘤病房,辽宁沈阳110016 [2]中国医科大学附属盛京医院内科风湿免疫病房,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2021年第7期722-730,共9页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省自然科学基金计划重点项目(20180540027)。

摘　　要：目的基于网络药理学探讨雷公藤多苷片主要成分与类风湿性关节炎之间的潜在作用机制。方法通过检索多个数据库,并结合类药性五原则,筛选出TG中的主要活性成分及作用的基因靶点;检索DrugBank、GeneCards和OMIM等数据库筛选RA的基因靶点,并经Uniprot数据库基因标准化处理,确定TG干预类风湿性关节炎的特异性靶点。将有效化合物和疾病靶点取交集,制作维恩图;绘制PPI网络图,将交集导入David数据库获取数据并进行GO分析和KEGG分析,以进一步分析其治疗关节炎的机制。结果雷公藤多苷片在数据库中共筛选到48种相应成分,根据类药性五原则,共筛选14种主要成分;从GeneCards和OMIM等数据库筛选RA疾病靶点636个。通过GO分析共获得43种分子功能,14种细胞组分,101种生物学过程。通过KEGG富集分析,一共得到66个富集通路。通过"中药-靶点-通路"网络图,将Degree排名前5的MAPK1、TNF、AR、MAPK8、IL-6作为雷公藤多苷片治疗RA的关键作用靶点。结论本研究运用网络药理学发现TG中14种活性成分,通过作用于人体内类风湿性关节炎相关靶点,相关的靶点和途径均涉及到炎症反应和免疫调节,来干预类风湿性关节炎疾病的进程。Objective To explore the relationship between the main components of tripterygium glycoside tablets(TG) and rheumatoid arthritis(RA) and the potential mechanism of action based on network pharmacology.Methods By searching multiple databases and combining the five principles of drug-like properties, the main active ingredients in TG and the gene targets of action were screened out;the gene targets of RA were screened through databases such as DrugBank, GeneCards and OMIM.In order to determine the specific target of TG intervention in rheumatoid arthritis, genes were standardized through the Uniprot database.And the genes were standardized by the Uniprot database.In this study, the effective compounds and the disease targets were taken to make a Venn diagram and the intersection was imported into the David database to obtain data.Furthermore, GO function enrichment analysis and KEGG pathway enrichment analysis were performed and the PPI network diagram was drawn to further analyze the mechanism of its treatment of arthritis.Results A total of 48 corresponding components of TG were screened in the database.According to the five principles of drug-like properties, a total of 14 main components were screened;636 RA disease targets were screened from databases such as GeneCards and OMIM.Through GO enrichment analysis, 43 main molecular functions(MF),14 main cell components(CC),and 101 main biological processes(BP) were obtained.Through KEGG enrichment analysis, a total of 66 enrichment pathways were obtained.Through the "Traditional Chinese Medicine-Target-Pathway" network diagram, the top 5 androgen receptor(AR),mitogen-activated protein kinase 1(MAPK1),target tumor necrosis factor(TNF),mitosis Pro-activated protein kinase 8(MAPK8),and interleukin-6(IL-6) in degree act as the key targets of TG in the treatment of RA.Conclusion This study uses network pharmacology to find that 14 main active ingredients in TG act on rheumatoid arthritis-related targets in the human body.Related targets and pathways are involved in infl

关 键 词：雷公藤多苷片 类风湿性关节炎 网络药理学 作用机制 富集分析 信号通路 

分 类 号：R917[医药卫生—药物分析学]