二甲双胍促进大鼠皮层神经元轴突生长的体外效应研究  

Metformin promotes axonal growth of rat cortical neurons in vitro

作　　者：管徒晨 滕龙 郭贝贝 刘炎[1] GUAN Tuchen;TENG Long;GUO Beibei;LIU Yan(Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,Nantong University,Nantong 226001)

机构地区：[1]南通大学教育部/江苏省神经再生重点实验室/神经再生协同创新中心,南通226001

出　　处：《南通大学学报（医学版）》2021年第3期199-202,F0002,共5页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(31970412);江苏省自然科学基金面上项目(BK20191446)。

摘　　要：目的:探究二甲双胍(metformin,MET)对原代皮层神经元轴突生长的效应,以寻找安全有效的促进神经元再生的药物,用于恢复中枢神经系统损伤。方法:原代培养大鼠胚胎18 d(E18)皮层神经元,加入不同浓度MET处理,采用免疫荧光观察神经元轴突生长状况,细胞计数试剂盒8(cell counting kit-8,CCK-8)检测细胞活力变化,利用硫酸软骨素蛋白多糖(chondroitin sulfate proteoglycans,CSPGs)构建神经元轴突生长抑制模型,免疫荧光观察MET是否可以缓解CSPGs的抑制作用。结果:100μmol/L浓度的MET能够有效促进原代皮层神经元轴突的生长和再生;相比于对照组,MET作用48 h能显著提升细胞活力;MET能够缓解CSPGs对神经元轴突生长的抑制作用。结论:MET可有效促进原代皮层神经元轴突生长及损伤轴突的再生,且能有效缓解CSPGs对皮层神经元生长的抑制作用。Objective:To investigate the effect of metformin(MET)on axonal growth and regeneration of primary cortical neurons and find a safe and effective drug to promote the regeneration of neurons,which can be used to recover the damage of central nervous system.Methods:Primary cortical neurons were cultured and treated with different concentrations of MET.The growth and regeneration of axons were observed by immunofluorescence staining and cell viability was measured by cell counting kit-8(CCK-8).Chondroitin sulfate proteoglycans(CSPGs)were used to establish an axonal growth inhibition model to observe whether MET could alleviate the inhibitory effect of CSPGs.Results:The growth and regeneration of primary cortical neuron axons was effectively promoted by MET at the concentration of 100μmol/L.Compared with the control group,MET significantly increased cell viability after 48 h stimulation.MET can alleviate the inhibitory effect of CSPGs on axon growth.Conclusion:MET can effectively promote the axon growth and regeneration of primary cortical neurons and alleviate the inhibitory effect of CSPGs on cortical neuron growth.

关 键 词：皮层神经元 二甲双胍 轴突生长 硫酸软骨素蛋白多糖 大鼠 

分 类 号：Q421[生物学—神经生物学]