赤芍对缺血性脑卒中大鼠PGC-1α/Nrf2通路促血管新生作用研究  

Study on the Promoting Angiogenesis of Paeoniae in Rats with Cerebral Ischemic Stroke by PGC-1α/Nrf2

作　　者：陈敏纯 闫抗抗 曹青[1] 谷建俐 郑洁[1] 刘生元 CHEN Min-chun;YAN Kang-kang;CAO Qing;GU Jian-li;ZHENG Jie;LIU Sheng-yuan(Department of Pharmacy,Xi’an No.3 Hospital,the Affiliated Hospital of Northwest University,Xi’an 710018,China)

机构地区：[1]西北大学附属医院/西安市第三医院药剂科,陕西西安710018

出　　处：《现代中药研究与实践》2021年第4期36-40,共5页Research and Practice on Chinese Medicines

基　　金：西安市科技计划项目[201805103YX11SF37(17)-7];西安市第三医院科研项目(Y20202008)。

摘　　要：目的探讨赤芍对缺血性脑卒中大鼠的促血管新生作用及分子机制。方法将SD大鼠随机分为假手术组,模型组,给药组低、中、高剂量组,分析大鼠神经功能,检测脑梗死容积,组织病理变化,脑微血管密度,细胞凋亡以及PGC-1α、Nrf2、VEGF、VEGFR-2蛋白表达。结果与模型组比较,赤芍可改善缺血性脑卒中大鼠的神经功能,缩小脑梗死灶,减轻组织病理损伤,减少细胞凋亡,增加脑微血管密度,促进脑组织PGC-1α、Nrf2、VEGF、VEGFR-2蛋白表达(P<0.05)。结论赤芍激活PGC-1α/Nrf2信号通路,诱导VEGF/VEGFR-2高表达,促进血管新生,发挥抗缺血性脑卒中作用。Objective To evaluate the efficacts of paeoniae on angiogenesis and its molecular mechanism in the ischemic stroke rats.Methods SD rats are randomly divided into sham group,model group and group with low,medium,high dose of paeoniae.The neurological function,cerebral infarction volume,histopathological,apoptosis,microvessel density and PGC-1α,Nrf2,VEGF,VEGFR-2 protein expression methods are used to study the efficacies of paeoniae in ischemic brain.Results Paeoniae is administration improved nerve function impairment,reduced cerebral infarction volume,ameliorated the histopathological damage,reduced the number of apoptotic cells,increased microvessel density and increased expression level of neuron PGC-1α,Nrf2,VEGF and VEGFR-2 protein(P<0.05).Conclusion Paeoniae could reduce cerebral ischemia injury by promoting angiogenesis through activating the signaling pathway of PGC-1α/Nrf2,and including the high expression of VEGF/VEGFR-2.

关 键 词：血管新生 赤芍 缺血性脑卒中 

分 类 号：R285.5[医药卫生—中药学]