姜黄素介导光动力疗法对具核梭杆菌的体外抑菌效应研究  

Antimicrobial effect of curcumin based photodynamic therapy on Fusobacterium nucleatum in vitro

作　　者：王冬青[1] 潘慧 张凤秋[2] WANG Dong-qing;PAN Hui;ZHANG Feng-qiu(Department of VIP Dental Service,Capital Medical University School of Stomatology,Beijing 100050,China)

机构地区：[1]北京首都医科大学口腔医学院特诊特需科,100050 [2]北京首都医科大学口腔医学院牙周科,100050 [3]西安交通大学口腔医院口腔综合科

出　　处：《北京口腔医学》2021年第4期203-206,共4页Beijing Journal of Stomatology

基　　金：北京东城区优秀人才项目(18-10-10);北京市属医院科研培育项目(PX2019056)。

摘　　要：目的体外分析蓝光LED联合姜黄素对具核梭杆菌的杀菌效果。方法实验对象具核梭杆菌标准菌株ATCC10953,分为三组:单独蓝光照射组(A组)、单纯姜黄素溶液组(B组)和光动力组(C组)。A组:采用蓝色LED光固化灯照射,功率密度0.3 W/cm^(2),照射时间20、40、60 s。B组:按照姜黄素溶液的浓度5、10、20、40μmol/L处理,不进行蓝光照射。C组:加入20μmol/L的姜黄素溶液后采用蓝光分别照射20、40、60 s;将0.12%葡萄糖酸氯己定处理的的菌液作为阳性对照;生理盐水处理的菌液作为阴性对照。所有实验后的菌液连续稀释,取10^(3)、10^(4)、10^(5)倍稀释菌液各10μl涂布在固体培养基上,厌氧培养1周后计算菌落形成单位(colony-forming units,CFU),取CFU的对数值进行统计分析。结果单独蓝光照射20、40、60 s后具核梭杆菌的菌落数减少,但无统计学差异(P>0.05)。与单独蓝光LED照射相比,姜黄素溶液在20μmol/L下单独使用时菌落数数量的减少较明显(P<0.05)。而20μmol/L姜黄素溶液处理联合60 s蓝光照射时杀菌效果最佳(P<0.05)。结论蓝光LED单独照射对具核梭杆菌的杀菌和生长抑制作用较弱,但联合姜黄素后杀菌效果增强。Objective To investigate the antimicrobial effect of curcumin-based PDT on Fusobacterium nucleatum in vitro.Methods Fusobacterium nucleatum 10953 suspension was irradiated with blue light-emitting diode(LED)(450-470 nm,output power density 1.2 W/cm^(2))for 20-60 s(6-18 J/cm^(2)),treated with curcumin(2 min),or subjected to a combination of curcumin treatment and blue LED irradiation(60 s,18 J/cm^(2)).A suspension of chlorhexidine gluconate(CHG)was used as the positive control.All bacterial suspensions used were serially diluted,plated,and incubated anaerobically.The numbers of colony-forming units(CFUs)were counted on day 7.Results BL irradiation at 6,12,and 18 J/cm^(2) alone reduced the number of CFUs of F.nucleatum,but the reduction was not statistically significant.Combined use of 18 J/cm^(2) blue LED irrigation and 20μmol/L curcumin treatment led to a 60%reduction of bacterial CFUs.Conclusions Within the limits of this study,a-PDT using a combination of blue LED and curcumin can inhibit the growth of F.nucleatum.

关 键 词：光动力疗法 姜黄素 蓝色发光二极管 具核梭杆菌 

分 类 号：R378[医药卫生—病原生物学]