精子DNA碎片指数对补救卵胞浆内单精子注射胚胎发育及临床结局的影响  

Effect of sperm DNA fragmentation index on embryonic development and clinical outcome of R-ICSI

作　　者：陈其桂 李大文[1] 成俊萍[1] 薛林涛[1] 李金燕 何泳志 谭卫红[1] CHEN Qigui;LI Dawen;CHENG Junping;XUE Lintao;LI Jinyan;HE Yongzhi;TAN Weihong(Center for Reproductive Medicine and Genetics,People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi,China;Graduate School,Guilin Medical University,Guilin 541004,Guangxi,China;Reproductive Medicine Center,Maternal and Child Health of Guangxi Zhuang Autonomous Region,Nanning 530003,Guangxi,China)

机构地区：[1]广西壮族自治区人民医院生殖医学与遗传中心,南宁530021 [2]桂林医学院研究生学院,广西桂林541004 [3]广西壮族自治区妇幼保健院生殖中心,南宁530003

出　　处：《中国性科学》2021年第8期1-4,共4页Chinese Journal of Human Sexuality

基　　金：国家自然科学基金(81360107);广西自然科学基金(2018GXNSFAA138112);广西卫健委科研课题(Z20170376);南宁市青秀区科技计划项目(2020030)。

摘　　要：目的探讨男性精子DNA碎片指数(DFI)对补救卵胞浆内单精子注射(R-ICSI)胚胎发育及临床结局的影响。方法选取2014年1月至2019年12月广西壮族自治区人民医院生殖医学与遗传中心行常规体外受精-胚胎移植(IVF-ET)失败后需行R-ICSI的79对不孕不育夫妇的临床资料进行回顾性分析。研究对象根据精子DFI分为精子DFI<30%组(n=63)和精子DFI≥30%组(n=16)。比较两组基线资料、胚胎发育情况及临床结局。结果两组不育年限、男方年龄、女方年龄、基础卵泡刺激素(FSH)、基础黄体生成素(LH)、窦卵泡数、绒毛膜促性腺激素(HCG)日子宫内膜厚度、获卵数比较,差异无统计学意义(P>0.05)。R-ICSI周期中,两组受精率、正常受精率、卵裂率、优胚率、种植率、临床妊娠率、活产率及流产率比较,差异无统计学意义(P>0.05)。结论精子DFI对R-ICSI患者胚胎发育及临床结局可能没有显著影响,需进一步研究论证。Objective To investigate the effect of the sperm DNA fragmentation index on embryonic development and clinical outcome of R-ICSI. Methods A total of 79 infertile couples who underwent R-ICSI after failure of routine in vitro fertilization-embryo transfer(IVF-ET) in Center for Reproductive Medicine and Genetics, People′s Hospital of Guangxi Zhuang Autonomous Region from January 2014 to December 2019 were selected as the study subjects, and their clinical data were retrospectively analyzed. According to sperm DFI, subjects were divided into sperm DFI<30% group(n=63) and sperm DFI≥30% group(n=16). Baseline data, embryo development and clinical outcomes were compared between the two groups. Results There was no statistical significance in baseline data such as infertility duration, male age, female age, basal serum FSH, basal serum LH, antral follicle count, ndometrial thickness of HCG, and number of eggs obtained between the two groups(P>0.05). During R-ICSI cycle, there were no significant differences in fertilization rate, normal fertilization rate, cleavage rate, good embryo rate, implantation rate, clinical pregnancy rate, live birth rate and abortion rate between the two groups(P>0.05). Conclusions Sperm DFI may have no significant effect on embryonic development and clinical outcome in patients with R-ICSI, which needs further study.

关 键 词：精子DNA碎片指数 补救卵胞浆内单精子注射 完全受精失败 胚胎发育 临床结局 

分 类 号：R697[医药卫生—泌尿科学]