miR-223通过靶向ARMCX1基因调控宫颈癌细胞的增殖和侵袭  

miR-223 regulates the proliferation and invasion of cervical cancer cells by targeting the ARMCX1 gene

作　　者：郭镜 陈丹 GUO Jing;CHEN Dan(Department of Rehabilitation Medicine,The Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China)

机构地区：[1]锦州医科大学附属第三医院康复科,辽宁锦州121001

出　　处：《西安交通大学学报（医学版）》2021年第5期681-686,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：辽宁省科技厅资助项目(No.201602326)。

摘　　要：目的探讨微小RNA(microRNA,miR)-223对宫颈癌siha细胞增殖和侵袭的影响及其作用机制。方法应用Real-time PCR技术检测宫颈癌患者癌组织及其癌旁组织中miR-223及Arm重复蛋白1(ARMCX1)mRNA的表达。建立miR-223过表达的siha细胞株(miR-223 mimic组),利用CCK8实验和Transwell实验观察对siha细胞生物学功能的影响。构建野生型和突变型ARMCX1双荧光素酶报告基因载体,并检测荧光素酶活性。Real-time PCR及Western blotting检测过表达miR-223的siha细胞的ARMCX1 mRNA和蛋白表达。结果与癌旁组织相比,宫颈癌组织中miR-223的表达明显降低(P<0.05),ARMCX1 mRNA表达明显升高(P<0.05)。与NC mimic组相比,miR-223 mimic组的宫颈癌siha细胞中miR-223的表达明显升高(P<0.05),且细胞增殖和侵袭能力明显降低(P<0.05)。而转染GV230-ARMCX1后,miR-223+GV230-ARMCX组细胞增殖和侵袭能力较miR-223+GV230-NC组增强(P<0.05)。双荧光素酶报告实验证实,miR-223可以直接靶向结合ARMCX1的3’UTR区序列,Real-time PCR和Western blotting结果显示,ARMCX1 mRNA和蛋白的表达降低(P<0.05)。结论miR-223在宫颈癌中低表达,并且可通过靶向ARMCX1基因抑制宫颈癌细胞的增殖和侵袭。Objective To investigate the effect of microRNA(miRNA,miR)-223 on the proliferation and invasion of siha cells in cervical cancer and its mechanism.Methods Fluorescent quantitative polymerase chain reaction(Real-time PCR)was used to detect the expression levels of miR-223 and arm duplication protein 1(ARMCX1)in cervical cancer tissues.Siha cell lines overexpressed by miR-223 were established,and the effects of overexpression of miR-223 on biological functions of siha cells were tested by CCK8 and Transwell test.Wild-type and mutant ARMCX1 double luciferase reporter vectors were constructed and luciferase activity was detected.The mRNA and protein expression levels of ARMCX1 were detected by Real-time PCR and Western blotting after overexpression of miR-223 in siha cells.Results Compared with that of adjacent tissues,the expression level of miR-223 in cervical cancer tissues was significantly decreased(P<0.05),and the mRNA expression lof ARMCX1 was significantly increased(P<0.05).Compared with the NC mimic group,the expression of miR-223 in miR-223 mimic group was significantly increased(P<0.05),and the cell proliferation and invasion ability were significantly reduced(P<005).However,after transfection with GV230-ARMCX1,cell proliferation and invasion abilities were enhanced in miR-223+GV230-ARMCX group compared with miR-223+GV230-NC group(P<0.05).The double luciferase reporting experiment confirmed that miR-223 could directly bind with ARMCX1’s 3’UTR region sequence,and Real-time PCR and Western blotting results showed that the expressions of ARMCX1 mRNA and protein were decreased(P<0.05).Conclusion miR-223 is underexpressed in cervical cancer and can inhibit the proliferation and invasion of cervical cancer cells by targeting ARMCX1 gene.

关 键 词：宫颈癌 增殖 侵袭 微小RNA-223 ARMCX1基因 

分 类 号：R711.74[医药卫生—妇产科学]