Kir4.1对少突胶质前体细胞分化的影响  

Effect of Kir4.1 on the Differentiation of Oligodendrocyte Precursor Cells

作　　者：柴智 肖晶晶 彭涛[2] 王丽娟 毕逢辰 王俊燕 海霞霞 丁万聪 李云鸿 王银[1,2] CHAI Zhi;XIAO Jingjing;PENG Tao;WANG Lijuan;BI Fengchen;WANG Junyan;HAI Xiaxia;DING Wancong;LI Yunhong;WANG Yin(Ningxia Key Laboratory of Craniocerebral Diseases,Yinchuan 750004,China;School of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏颅脑疾病重点实验室,银川750004 [2]宁夏医科大学基础医学院,银川750004

出　　处：《宁夏医科大学学报》2021年第8期777-781,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(82060223);宁夏自然科学基金(2020AAC03143,2020AAC03150);宁夏大学生创新创业训练项目(S202010752039,S202010752032)。

摘　　要：目的探究内向整流钾离子通道4.1(inwardly rectifying K channel 4.1,Kir4.1)对少突胶质前体细胞(oligodendrocyte precursor cell,OPC)分化的影响。方法体外分别培养OPC和少突胶质细胞(oligodendrocyte,OL),qRT-PCR检测OPC和OL中Kir4.1的mRNA表达情况,Western blot检测OPC与OL中Kir4.1的表达水平。将OPC细胞分为对照组及Kir 4.1阻断剂地昔帕明(desipramine,Des)处理的Des处理组,Western blot检测髓鞘碱性蛋白(myelin basic protein,MBP)的表达;免疫荧光细胞染色法检测对照组和Des处理组中少突胶质细胞转录因子2(oligodendrocyte transcription factor 2,Olig2)与MBP的表达水平。结果Kir4.1在OPC和OL中均有表达,与OPC比较,OL中Kir4.1的mRNA和蛋白的表达量均增加(P均<0.01);Des处理组MBP的表达水平低于对照组(P<0.01);免疫荧光结果显示,Des处理组MBP的表达低于对照组(P<0.01)。结论在OPC的分化过程中,Kir4.1发挥着非常重要的作用,在体外阻断Kir4.1会抑制OPC的分化。Objective To investigate the effect of inwardly rectifying k channel 4.1(Kir4.1)on the differentiation of oligodendrocyte precursor cells(OPC).Methods OPC and oligodendrocytes(OL)were cultured in vitro.The mRNA expression of Kir4.1 in OPC and OL was detected by qRT-PCR.The expression of Kir4.1 protein in OPC and OL was detected by Western blot.OPC were divided into control group and Des group treated with Kir4.1 blocker desipramine(Des).The expression of myelin basic protein(MBP)was detected by Western blot,and the expression of oligodendrocyte transcription factor 2(Olig2)and MBP in control group and Des processing group was detected by immunofluorescence staining.Results Kir4.1 was expressed in both OPC and OL.Compared with OPC,the expression of mRNA and protein of Kir4.1 in OL was significantly increased(P all<0.01),and the expression level of MBP in Des processing group was significantly lower than that in control group(P<0.01).Immunofluorescence results showed that the expression of MBP in Des processing group was much lower than that in control group(P<0.01).Conclusion Kir4.1 plays a very important role in the differentiation of OPC.Blocking Kir4.1 in vitro can inhibit the differentiation of OPC.

关 键 词：内向整流钾通道4.1 少突胶质细胞 少突胶质前体细胞 

分 类 号：R332[医药卫生—人体生理学]