老年小鼠海马离体脑片长时程增强方法的建立  

Establishment of long-term potentiation method in hippocampal slices from aged mice

作　　者：李华 朱改只 周珊 徐雅琪 崔秀玉 周岩 王仁喜[2] 粟文婷 LI Hua;ZHU Gaizhi;ZHOU Shan;XU Yaqi;CUI Xiuyu;ZHOU Yan;WANG Renxi;SU Wenting(Core Facilities,Capital Medical University,Beijing 100069,China;Collaborative Innovation Center forBrain Disorders,Laboratory of Brain Disorders,Ministry of Science and Technology,Beijing Institute ofBrain Disorders,Capital Medical University,Beijing 100069,China;Department of Neurobiology,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学中心实验室,北京100069 [2]首都医科大学脑重大疾病研究中心,北京脑重大疾病研究院,北京市脑重大疾病重点实验室-省部共建国家重点实验室培育基地,脑重大疾病防治省部共建协同创新中心,北京100069 [3]首都医科大学神经生物学系,北京100069

出　　处：《实验技术与管理》2021年第8期51-54,60,共5页Experimental Technology and Management

基　　金：国家自然科学基金委员会青年科学基金项目(81704151);北京市教委科技一般项目(KM201710025014);国家自然科学基金委员会面上项目(31770956,82071758)。

摘　　要：老年小鼠离体电生理技术的一个难点是保证实验动物脑片神经元的活性。该研究参考国内外相关文献并进行优化,摸索出能制备高质量活性的老年小鼠急性脑片的实验体系。在搭配无创的64通道平面微电极阵列记录系统的基础上,建立了一套适合记录老年小鼠离体海马脑片长时程增强的方法和平台,为老年学习记忆能力的研究提供了一个强有力的实验工具。该方法操作简便、成功率高,制备的急性脑片也适用于膜片钳记录的研究。One of the difficulties in the electrophysiological technique of aged mice in vitro is to ensure the activity of neurons in brain slices from experimental animals.This study refers to the relevant literature at home and abroad and optimizes the experimental system to prepare high-quality and active acute brain slices of aged mice.Based on the noninvasive 64 channel planar microelectrode array recording system,a set of methods and platforms for recording the long-term potentiation of hippocampal slices in old mice is established,which provides a powerful experimental tool for the study of learning and memory ability of the elderly.This method is simple and has high success rate,and the prepared acute brain slices are also suitable for patch clamp recording.

关 键 词：神经电生理 老年小鼠急性脑片 平面微电极阵列记录系统 长时程增强 

分 类 号：Q189[生物学—神经生物学] Q424[生物学—普通生物学]