基于空心二硫化钼纳米球/金纳米花的神经元特异性烯醇化酶光电化学免疫传感器  

A Photoelectrochemical Immunosensor for Neuron Specific Enolase Based on Hollow Molybdenum Disulfide Nanoballs and Gold Nanoflowers

作　　者：刘仁喜 王炎英[1] 刘蔻 黄雅娇 李海燕[1] 李春涯[1] LIU Renxi;WANG Yanying;LIU Kou;HUANG Yajiao;LI Haiyan;LI Chunya(College of Chemistry and Materials Science,South-Central University for Nationalities,Wuhan 430074;School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,325035)

机构地区：[1]中南民族大学化学与材料科学学院,湖北武汉430074 [2]温州医科大学药学院,浙江温州325035

出　　处：《分析科学学报》2021年第4期529-535,共7页Journal of Analytical Science

基　　金：国家自然科学基金(No.21874157,21675175,22004133);湖北省科技创新专项重大项目(No.2017ACA172)。

摘　　要：以钼酸铵为钼源,硫脲为硫源,1-丁基-3-甲基咪唑四氟硼酸盐离子液体为溶剂和形貌控制剂,采用溶剂热法制备空心MoS2纳米球(MoS2NBs)。以乙二醇为溶剂,三乙醇胺为还原剂,采用水浴法制备金纳米花(AuNFs)。将空心(MoS2NBs)与AuNFs组装后修饰到玻碳电极表面,进一步固定神经元特异性烯醇化酶(NSE)的识别抗体,再经戊二醛交联、牛血清白蛋白封闭非特异性结合位点,制备NSE的位阻效应无标记光电化学免疫传感器。在最优化条件下,该传感器对NSE的线性响应范围为0.004~2.5 ng·mL^(-1),检测限(S/N=3)为1.83 pg·mL^(-1)。该光电化学免疫传感器对NSE检测还具有良好的选择性、稳定性、重现性以及较高的灵敏度。当用于临床血清样品中NSE的检测时,其结果与电化学发光法一致,表明该光电化学免疫传感器具有潜在的临床应用价值。Using ammonium molybdate as the molybdenum source,thiourea as the sulfur source,and 1-butyl-3-methylimidazole tetrafluoroborate ionic liquid as solvent and morphology controlling reagent,hollow molybdenum disulfide nanoballs(MoS2NBs)were prepared using a solvothermal method.Using ethylene glycol as the solvent and triethanolamine asreductant,gold nanoflowers(AuNFs)were facilely prepared through water-bath heating.AuNFs were integrated with MoS2NBs to form an AuNFs/MoS2 nanocomposite.AuNFs/MoS2 nanocomposite was then drop coated onto a glassy carbon electrode surface to construct a photoactive interface for immobilizing neuron specific enolase antibody(anti-NSE).After being stabilizing through a cross-linking reaction with glutaraldehyde and blocking non-specific binding sites with bovine serum albumin,a photoelectrochemical immunosensor was successfully fabricated for NSE determination.Under the optimized conditions,the photocurrent responses show a linear relationship to NSE concentration in the range from 0.004 to 2.5 ng·mL-1 with a detection limit of 1.83 pg·mL-1(S/N=3).The photoelectrochemical immunosensor provides good selectivity,stability,reproducibility and high sensitivity for NSE assay.The photoelectrochemical immunosensor was also successfully employed for determining NSE in clinical serum samples with satisfied results which are well consistent with that obtained from electrochemiluminescence immunoassay,indicating the photoelectrochemical immunosensor possesses promising values in tumor diagnosis by monitoring NSE concentration.

关 键 词：二硫化钼 金纳米花 神经元特异性烯醇化酶 光电化学免疫传感 

分 类 号：O657[理学—分析化学]