机构地区:[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学化学化工与资源利用学院,黑龙江哈尔滨150040 [3]东北林业大学林学院,黑龙江哈尔滨150040
出 处:《经济林研究》2021年第3期25-31,98,共8页Non-wood Forest Research
基 金:林业公益性行业科研专项经费资助项目(201504701-2);中央高校基本科研业务费专项资金资助项目(2572020BU02)。
摘 要:【目的】根际是土壤—植物生态系统物质交换的活跃界面,研究杜仲根际土壤的变化差异,为今后杜仲的栽培管理提供依据。【方法】以杜仲根际土壤(GJ)与非根际土壤(FG)为研究材料,测定其土壤的理化性质变化,分析土壤微生物功能多样性和微生物功能基因表达的变化。【结果】杜仲GJ碳、氮、磷指标高于FG,其中GJ全氮含量比FG高30.81%,GJ碱解氮含量是FG的1.39倍,但其GJ硝态氮含量比FG低,FG的酸性程度、电导率显著高于GJ(P<0.05),化学计量比(C/N、C/P、N/P)之间差异不显著。杜仲GJ微生物含量比FG高且能较早利用碳源,GJ总碳源的利用能力(AWCD)、物种均匀度指数(U)均大于FG(P<0.05)。杜仲GJ真菌ITS、nifH、amoA-AOA和nosZ基因表达量均显著高于FG,GJ Acidobacteria基因以及phoD基因显著低于FG(P<0.05),Thermomicrobia、nifH、nirS、amoA-AOB、narG基因的拷贝数较低。冗余分析结果表明,杜仲土壤微生物与土壤理化指标变化存在相关性,杜仲GJ的主要影响因子是phoD和Acidobacteria基因,杜仲FG的主要影响因子是nirS基因与phoD基因,【结论】杜仲根际与非根际土壤理化性质间存在着显著的差异,其土壤微生物含量在其中起着至关重要的作用。【Objective】Rhizosphere is the active interface of material exchange between soil and plant ecosystem.The study on the variation of the rhizosphere soil of Eucommia ulmoides can provide the basis for the cultivation of E.ulmoides in the future.【Method】The rhizosphere(GJ)and non rhizosphere(FG)soil of E.ulmoides were used as research materials to determine the changes of soil physical and chemical properties,and analyze the changes of soil microbial functional diversity and microbial functional gene expression.【Result】The indexes of carbon,nitrogen and phosphorus of E.ulmoides GJ were higher than those of FG.The total nitrogen content of GJ was 30.81%higher than that of FG,and the alkali hydrolyzed nitrogen content of GJ was 1.39 times higher than that of FG,but the nitrate nitrogen content of GJ was lower than that of FG.The acidity and electrical conductivity of FG were significantly higher than that of GJ(P<0.05),and there was no significant difference between stoichiometric ratios(C/N,C/P,N/P).The microbial content of GJ in E.ulmoides was higher than that of FG,and it could use carbon source earlier.The total carbon utilization capacity(AWCD)and species evenness index(U)of GJ were higher than those of FG(P<0.05).The expression levels of ITs,nifH,amoA-AOA and nosZ genes in GJ were significantly higher than those in FG,and the Acidobacteria and phoD genes of GJ were significantly lower than those of FG(P<0.05).The copy numbers of Thermomycrobia,nifH,nirS,amoA-AOB and narG genes were lower.The results of redundancy analysis showed that there was correlation between soil microorganism and soil physicochemical indexes.The main influencing factors of GJ were phoD and Acidobacteria genes,and the main influencing factors of FG were nirS gene and phoD gene.The results showed that the correlation between the expression of phoD gene and the physical and chemical indexes of E.ulmoides was the highest,indicating that phoD gene was the most important factor affecting the physical and chemical indexes of E.ulmoide
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