环境相关浓度的睾酮对秀丽线虫的生殖毒性作用  

Reproductive toxicity of environmentally relevant concentrations of testosterone on Caenorhabditis elegans

作　　者：马令怡 李维溪 尹洁晨 朱光灿 刘冉 MA Lingyi;LI Weixi;YIN Jiechen;ZHU Guangcan;LIU Ran(Key Laboratory of Environmental Medicine of Engineering and Ministry of Education,School of Public Health,Southeast University,Nanjing,Jiangsu 210009,China;Key Laboratory of Pesticide Environmental Assessment and Pollution Control,Nanjing Institute of Environmental Sciences,Ministry of Ecology and Environment,Nanjing,Jiangsu 210042,China)

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室,江苏南京210009 [2]生态环境部南京环境科学研究所,国家环境保护农药环境评价与污染控制重点实验室,江苏南京210042

出　　处：《环境与职业医学》2021年第8期910-914,共5页Journal of Environmental and Occupational Medicine

基　　金：江苏省域城乡统筹供水技术集成与综合示范项目(2014ZX07405002);江苏省研究生科研与实践创新计划项目(SJCX20_0060);中央高校基本科研业务费专项资金资助(3225002106D)。

摘　　要：[背景]睾酮是一种重要的雄激素,由于人类生产生活活动造成它在环境中的不同程度暴露,可能影响生态环境中多种生物的生殖能力。[目的]以秀丽隐杆线虫为模式生物,探讨环境相关浓度的睾酮的生殖毒性作用。[方法]根据睾酮在环境中实际暴露水平选取有代表性的环境相关质量浓度(简称为浓度,1 ng·L^(-1)、10 ng·L^(-1)、100 ng·L^(-1)、1μg·L^(-1)、10μg·L^(-1))作为本研究的暴露浓度,将同步化的L1期野生型N2线虫暴露于不同浓度的睾酮及M9溶剂(对照组)中,20℃下恒温培养染毒48 h。观察各组生殖毒性指标,包括后代数目、世代时间、性腺发育阶段、畸形现象、生殖细胞数目。[结果]野生型N2秀丽隐杆线虫自L1期暴露于睾酮48 h后,与对照组(219.70±27.99)相比,10 ng·L^(-1)~10μg·L^(-1)组后代数目减少(分别为186.90±19.68,P=0.009;182.60±25.33,P=0.003;178.20±14.46,P=0.001;159.20±28.64,P<0.001)(F=7.24,P<0.001);10μg·L^(-1)组与对照组相比后代数目减少了27.5%。与对照组[(71.85±3.49)h]相比,100 ng·L^(-1)组[(81.70±6.26)h,P=0.001]、1μg·L^(-1)组[(79.28±4.83)h,P=0.015]、10μg·L^(-1)组[(79.54±3.92)h,P=0.011]世代时间延长(F=3.97,P=0.005);100 ng·L^(-1)组世代时间延长最多,为9.85 h。对性腺发育阶段的观察发现,随染毒浓度的增高,性腺发育逐渐受到抑制,各个剂量组中的成虫比例从对照组的(63.82±16.37)%逐渐下降至(53.95±1.03)%、(41.39±8.57)%、(26.79±1.31)%、(20.16±1.94)%、(8.00±2.03)%。在100 ng·L^(-1)及以上染毒组线虫中观察到畸形现象,包括排卵孔畸形、子宫内卵未受精、虫袋、性腺发育异常。与对照组(249.40±23.09)相比,10 ng·L^(-1)~10μg·L^(-1)组生殖细胞数目减少[分别为(224.50±28.48,P=0.048)、(215.20±27.28,P=0.005)、(194.30±26.78,P<0.001)、(181.80±19.45,P<0.001)](F=10.64,P<0.001);10μg·L^(-1)组生殖细胞数目较对照组减少了27.1%。[结论]环境相关浓度的雄激素睾酮�[Background]Testosterone is an important androgen.However,human production and living activities have led to its exposure to varying degrees in the environment,which may affect the reproductive ability of many organisms in the ecological environment.[Objective]Caenorhabditis elegans(C.elegans)is used as a model organism to investigate the reproductive toxicity of testosterone at environmentally relevant concentrations.[Methods]According to the actual concentration of testosterone in the environment,synchronized L1-stage wild-type N2 nematodes were exposed to representative environmentally relevant concentrations(1 ng·L^(-1),10 ng·L^(-1),100 ng·L^(-1),1μg·L^(-1),10μg·L^(-1))of testosterone and M9 solvent(control)at 20℃for 48 h.Brood size,generation time,gonadal development stage,malformation,and the number of germ cells were observed among the groups.[Results]After the wild-type N2 C.elegans being exposed to testosterone for 48 h in the L1 stage,compared with the control group(219.70±27.99),the brood sizes of C.elegans were significantly decreased in the 10 ng·L^(-1) to 10μg·L^(-1) groups(186.90±19.68,P=0.009;182.60±25.33,P=0.003;178.20±14.46,P=0.001;159.20±28.64,P<0.001,respectively)(F=7.24,P<0.001),and in the 10μg·L^(-1) group the brood size decreased by 27.5%.Compared with the control group[(71.85±3.49)h],the generation time was significantly prolonged in the 100 ng·L^(-1) group[(81.70±6.26)h,P=0.001],1μg·L^(-1) group[(79.28±4.83)h,P=0.015],and 10μg·L^(-1) group[(79.54±3.92)h,P=0.011](F=3.97,P=0.005),and was longest in the 100 ng·L^(-1) group where the generation time was prolonged by 9.85 h.In terms of gonadal development stage,as the concentration of testosterone increased,the development of gonads was gradually inhibited,and the proportion of adult worms to total nematodes gradually decreased from(63.82±16.37)%in the control group to(53.95±1.03)%,(41.39±8.57)%,(26.79±1.31)%,(20.16±1.94)%,and(8.00±2.03)%in each dose group,respectively.Malformations were observed in the 100 n

关 键 词：睾酮 秀丽隐杆线虫 生殖毒性 性腺发育迟缓 生殖细胞减少 

分 类 号：R123.1[医药卫生—环境卫生学]