稻虱缨小蜂响应水稻挥发物的性二型性特征  

Sexual differences in the response of the parasitoid wasp Anagrus nilaparvatae to rice volatiles

作　　者：黄庭发 马莹[2] 汤冰洁 王冰洋 曾柔仙 周强 张古忍[1] HUANG Ting-Fa;MA Ying;TANG Bing-Jie;WANG Bing-Yang;ZENG Rou-Xian;ZHOU Qiang;ZHANG Gu-Ren(School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;School of Agriculture,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]中山大学生命科学学院,广州510275 [2]中山大学农学院,广州510275

出　　处：《应用昆虫学报》2021年第4期876-884,共9页Chinese Journal of Applied Entomology

基　　金：广州市重点项目专题(201804020062);广东省现代农业产业技术体系创新团队(2020KJ113)。

摘　　要：【目的】比较稻虱缨小蜂Anagrus nilaparvatae雌雄蜂对水稻挥发物的电生理响应,明确寄生蜂嗅觉感受能力的性别差异,为提高寄生蜂在害虫生物防治中的应用效率提供依据。【方法】建立微小触角电位检测方法,测量雌雄蜂对不同类别的9种水稻挥发物的触角电位反应,对具有差异反应的挥发物进行梯度浓度刺激实验。【结果】电生理测试中,雄蜂对反-2-己烯醛的反应最强,对反-石竹烯的反应最弱,反应顺序从大到小为醛、酯、醇、萜;雌蜂对顺-茉莉酮的反应最强,对芳樟醇的反应最弱。测试的9种水稻挥发物中,6种挥发物在雌雄间存在显著的响应差异。在梯度浓度测试中,雄蜂对顺-柠檬烯、芳樟醇和顺茉莉酮3种挥发物的浓度变化没有明显的电生理响应差异,而对反-石竹烯、橙花叔醇和反-2-己烯醇产生明显电生理反应;雌蜂对6种挥发物的浓度变化都产生明显的电生理响应,其中,萜烯类物质芳樟醇、反-石竹烯和橙花叔醇在测试的最低浓度(0.01 g/L)时的电生理反应最强。【结论】稻虱缨小蜂的嗅觉感受能力在雌雄间存在明显的差异,且雌蜂对水稻挥发物的电生理反应较雄蜂灵敏。挥发物浓度在范围0.01-100 g/L内,通过稻虱缨小蜂对不同浓度下EAG值的差异,可以初步判断不同浓度的挥发物存在不同生态功能。[Objectives]To clarify sexual differences in the response of the parasitoid wasp Anagrus nilaparvatae to rice volatiles in order to improve the effectiveness of using parasitic wasps as biological control agents.[Methods]A potentiometric,micro-antennae detection method was developed to measure the EAG responses of female and male A.nilaparvatae to 9 different kinds of rice volatiles.Gradient concentration stimulation experiments were also performed to detect significant gender differences in the response to specific chemicals.[Results]The largest peak amplitudes in males were recorded in response to trans-2-hexenal and the smallest to trans-caryophyllene.Responses of males to aldehydes,esters,alcohols and terpenes ranged from large to small.For females,the highest response was to cis-jasmone and the lowest to linalool.Six of the 9 rice volatiles tested elicited significantly different responses in males and females.In the gradient concentration experiment,only 3 of the 6 volatiles tested(trans-caryophyllene,nerolidol and trans-2-hexenol)elicited a significant response from males,whereas all 6 compounds elicited a significant response from females.The terpenoids linalool,trans-caryophyllene and nerolidol,elicited the strongest responses at a concentration of 0.01 g/L,which was the lowest concentration used in experiments.[Conclusion]There are significant sexual differences in the responses of A.nilaparvatae to rice volatiles;females respond to a greater range of volatiles than males.EAG responses to rice volatiles were detected within the concentration range of 0.01-100 g/L,which could be a preliminary indication that differences in EAG responses to volatiles reflect different ecological functions.

关 键 词：稻虱缨小蜂 水稻挥发物 触角电位反应 性二型性 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治] S476.3[农业科学—植物保护]