不同药剂处理对番茄连作培养基质微生物群落的影响  

Effects of different pharmaceutical treatments on microbial community of tomato continuous culture matrix

作　　者：闫思华 王慧茹 李建设[1] 高艳明[1] YAN Si-hua;WANG Hui-ru;LI Jian-she;GAO Yan-ming(College of Agriculture Ningxia University,Yinchuan Ningxia 750021)

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《中国土壤与肥料》2021年第4期273-282,共10页Soil and Fertilizer Sciences in China

基　　金：宁夏回族自治区重点研发计划项目(2019BBF02008)。

摘　　要：为探讨连作基质微生物群落对不同消毒药剂的响应,设置6个处理,即施入枯草芽孢杆菌+地衣芽孢杆菌(T1)、恶霉灵+氯溴异氰尿酸(T2)、恶霉灵+乙酸铜(T3)、2.2%酒精(T4)、甲醛(T5)5种药剂消毒组合,以清水处理为对照(CK),依托Illumina MiSeq高通量测序平台,研究不同药剂处理对基质细菌和真菌群落组成及多样性的影响。结果表明:不同药剂消毒后基质细菌和真菌群落多样性下降,与CK相比,T4处理显著降低了细菌OTU序列、丰富度指数(Chao 1和Ace指数)和多样性指数(Shannon指数),T3~T5处理显著降低真菌OTU序列(40.87%~47.49%),Chao 1指数(40.58%~46.58%)和Ace指数(41.16%~46.51%)。不同药剂施入影响群落结构及组成,其中绿弯菌门、厚壁菌门、放线菌门、变形菌门和子囊菌门相对丰度达到10%以上,与CK相比,T5处理后的厚壁菌门、放线菌门、子囊菌门相对丰度提高,厚壁菌门中的耐受性细菌芽孢杆菌科相对丰度降幅较小,子囊菌门中的曲霉科相对丰度提高29.14%,表现为T4>T5>T3>T2>CK>T1。本研究表明,不同药剂消毒改变了连作基质细菌和真菌群落组成,降低菌群多样性,且甲醛处理后最为明显。In order to investigate the response of continuous cropping matrix microbial community to different disinfection agents,six treatments were set up in this study,including the combination of Bacillus subtilis and Bacillus licheniformis(T1),Hymexazol and Chlorobromoisocyanurate(T2),Hymexazol and Copper acetate(T3),2.2% Alcohol(T4),Formaldehyde(T5),and control with water treatment(CK).Based on Illumina MiSeq high-throughput sequencing platform,the effects of different treatments on the composition and diversity of matrix bacterial and fungal communities were studied.The results showed that the diversity of host bacterial and fungal communities decreased after disinfection with different agents.Compared with CK,T4 treatment significantly decreased bacterial OTU sequence,richness index(Chao 1 and Ace index)and diversity index(Shannon index),T3 ~ T5 treatment significantly decreased fungal OTU sequence(40.87% ~ 47.49%),Chao 1 index(40.58% ~ 46.58%)and Ace index(41.16% ~ 46.51%).The relative abundance of Chloroflexi,Firmicutes,Actinobacteri,Proteobacteria and Ascomycota reached more than 10%.Compared with CK,the relative abundance of Firmicutes,Actinobacteri and Ascomycota increased in T5 treatment,the relative abundance of tolerant bacteria Bacillaceae in the Firmicutes phylum decreased slightly,and the relative abundance of Aspergillaceae in the Ascomycota increased by 29.14%,showing as T4>T5>T3>T2>CK>T1.This study showed that different agents disinfection changed the composition of continuous substrate bacterial and fungal community,reduced the diversity of flora,with the mose obvious changes in Formaldehyde treatment.

关 键 词：药剂消毒 番茄土传病害 基质微生物群落 高通量测序 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]