外周血造血干细胞单采影响因素及时机预测的探讨  

Influencing factors and timing prediction of hematopoietic stem cell collection in peripheral blood

作　　者：李少文[1] 朱展鸿 郭楚霞 高旑鑫 王刚 彭永正[1] 陆志刚[1] LI Shaowen;ZHU Zhanhong;GUO Chuxia;GAO Yixin;WANG Gang;PENG Yongzheng;LU Zhigang(Department of Transfusion,Zhujiang Hospital of Southern Medical University,Guangzhou 510280,China;Departmentof Transfusion,the Southern Theater General Hospital of the Chinese People′s Liberation Army)

机构地区：[1]南方医科大学珠江医院输血科,广东广州510282 [2]南部战区总医院输血科

出　　处：《中国输血杂志》2021年第8期843-846,共4页Chinese Journal of Blood Transfusion

摘　　要：目的研究捐献者血常规参数对外周血造血干细胞采集效果的影响,及如何通过这些参数预测采集时机。方法收集2018年1月至2020年8月本院使用血细胞分离机进行外周血造血干细胞采集的249例捐献者捐献前血常规参数及采集后产品的单个核细胞总数,以产品每升循环血量单个核细胞总数为采集效果的主要评价指标,研究其与采集前血常规参数间是否存在相关关系,并对相关影响因素分析采用多元线性回归分析。收集2020年9~10月本院进行外周血造血干细胞采集捐献者血常规参数代入方程,所得值为预测值,并与实际产品所得的实际值使用独立样本t检验进行验证。结果通过相关分析得出血红蛋白、红细胞计数、红细胞比容、白细胞计数、中性粒细胞、淋巴细胞、单核细胞和血小板计数等参数与每升循环血量单个核细胞总数存在统计学上相关(P<0.05)。作图得出淋巴细胞、单核细胞、血小板计数和白细胞计数与每升循环血量单个核细胞总数存在明显线性关系。把每升循环血量单个核细胞总数设为(Y),以淋巴细胞(X_(1))、单核细胞(X_(2))、血小板计数(X3)、白细胞计数(X4)、和中性粒细胞等相关变量为因变量进行逐步多元线性回归,得出回归方程为:Y=9.814+3.131X_(1)+1.666X_(2)+0.020X3+0.124X4。验证结果,预测值与计算值间不存在统计学差异(P>0.05)。结论使用捐献者的淋巴细胞、单核细胞、血小板计数和白细胞计数这四个采集前血常规数据,可有效预测采集效率,从而选择最合适的采集时机。Objective To explore the effects of blood routine parameters on the peripheral blood hematopoietic stem cell collection of healthy donors,and predict collection timing based on these parameters.Methods The blood routine parameters pre-donation and the total number of mononuclear cells post-donation of 249 donors who applied blood cell separator to collect peripheral blood hematopoietic stem cells in our hospital from January 2018 to August 2020 were collected.Taking total nucleated cells of circulating blood per litre as the main evaluation index,and its collection with blood routine parameters pre-collection was analyzed.The relevant influencing factors were analyzed using multiple linear regression analysis.The blood routine parameters of healthy donors who donated peripheral blood hematopoietic stem cells in our hospital from September 2020 to October 2020 were substituted into the equation to obtain the predicted values,which were then compared with the actual values obtained from actual product using t test for verification.Results The analysis showed that the parameters of Hb,RBC,Hct,leukocyte count,neutrophil,lymphocyte,monocyte and Plt were statistically correlated with the total number of mononuclear cells of circulating blood per liter volume(P<0.05).There was a linear relationship between lymphocyte,monocyte,Plt and leukocyte count and the total number of mononuclear cells of circulating blood per liter.The total number of mononuclear cells of circulating blood per liter was set to(Y),and the variables such as lymphocyte(X_(1)),monocyte(X_(2)),Plt(X3),leukocyte count(X),and neutrophil were used as dependent variables for multiple linear regression,and the equation was:Y=9.814+3.131X_(1)+1.666X_(2)+0.020X3+0.124X4.There was no statistical difference between the predicted value and the calculated value(P>0.05).Conclusion The blood routine parameters of lymphocyte,monocyte,platelet count and leukocyte count of donors before collection can effectively predict the collection efficiency,therefore help predic

关 键 词：捐献者 血常规 采集效率 预测时机 

分 类 号：R457.7[医药卫生—治疗学]