核激素受体家族在皮肤鳞状细胞癌细胞中的表达  

Expressions of nuclear hormone receptor superfamily in skin squamous cell carcinoma A431 cells

作　　者：刘林红 张华 张颖[1] 胡美玲[1] 张斌 LIU Lin-hong;ZHANG Hua;ZHANG Ying;HU Mei-ling;ZHANG Bin(Department of Dermatology,Chongqing Hospital of Traditional Chinese Medicine Chongqing 400011,China;Centers for Disease Control and Prevention of Tianqiao District,Jinan,Shandong 250031,China)

机构地区：[1]重庆市中医院皮肤科,重庆400011 [2]山东省济南市天桥区疾病预防控制中心,山东济南250031

出　　处：《皮肤病与性病》2021年第4期459-462,共4页Dermatology and Venereology

基　　金：重庆市自然科学基金资助项目(cstc2018jcyjAX0370)。

摘　　要：目的通过研究核激素受体家族成员在皮肤鳞癌A431细胞中的表达,分析核激素受体家族成员在皮肤鳞癌发生机制中的作用。方法使用PCR Array技术分别检测人皮肤角质形成细胞和皮肤鳞状细胞癌细胞中核激素受体成员mRNA的相对表达水平。使用2^(-△△CT)方法计算皮肤鳞状细胞癌细胞中核激素受体mRNA的相对变化。结果核激素受体在角质形成细胞以及皮肤鳞状细胞癌细胞中均表达,RXR二聚体家族成员如VDR、RXR、RAR、PPAR以及LXR等表达量较高。A431细胞中多种受体的mRNA表达水平较正常角质形成细胞明显升高,尤其是RXR二聚体家族成员。结论在皮肤鳞状细胞癌细胞中存在大量核激素受体家族成员,尤其是RXR异构二聚体类成员的高表达,这些成员的异常表达可能在肿瘤发生发展中发挥作用,也可能为肿瘤的治疗提供靶点。Objective To detect nuclear hormone receptors expression in skin squamous cell carcinoma A431 cells and analyze its role in the pathogenesis of skin squamous cell carcinoma.Methods PCR Array was used to detect the mRNA expression levels of nuclear hormone receptor members in keratinocytes and skin squamous cell carcinoma cells.The relative changes of nuclear hormone receptor mRNA in skin squamous cell carcinoma cells were calculated by 2^(-△△CT) Results Nuclear hormone receptor expression was detected both in keratinocytes and in skin squamous cell carcinoma cells.The expression of RXR dimer family members such as VDR,RXR,RAR,PPAR and LXR was high.The mRNA expression levels of multiple receptors,especially the members of the RXR dimer family,were significantly higher in A431 cells than in normal keratinocytes.Conclusion High expression of a large number of nuclear hormone receptors,especially the members of the RXR isomeric dimer family might play a role in development or progression of skin squamous cell carcinoma,which might also provide potential targets of skin tumor therapy.

关 键 词：核激素受体 鳞状细胞癌 

分 类 号：R715.02[医药卫生—妇产科学] R730.261[医药卫生—临床医学]